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epsA [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]
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epsA [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

extracellular polysaccharide synthesis, putative transmembrane modulator of EpsB activity, might activate EpsB autophosphorylation and substrate phosphorylation
locus
BSU34370
pI
6.07
mw
25.00 kDa
function
biofilm formation
product
unknown
essential
no
synonyms
yveK
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.1|Matrix polysaccharide synthesis]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • [SW|Categories] containing this gene/protein

  • [SW|protein modification], [SW|biofilm formation], [SW|membrane proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|AbrB regulon], [SW|RemA regulon], [SW|SinR regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    3,529,151 -> 3,529,855

    The protein

    Protein family

  • cpsC/capA family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|search|TkmA]

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot)

    • [SW|Interactions]

  • [protein|search|EpsA]-[protein|search|EpsB] [Pubmed|24493247]

    • Expression and Regulation

    Operon

  • ''[protein|search|epsA]-[protein|search|epsB]-[protein|search|epsC]-[protein|search|epsD]-[protein|search|epsE]-[protein|search|epsF]-[protein|search|epsG]-[protein|search|epsH]-[protein|search|epsI]-[protein|search|epsJ]-[protein|search|epsK]-[protein|search|epsL]-[protein|search|epsM]-[protein|search|epsN]-[protein|search|epsO]'' [Pubmed|15661000]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|15661000]

    • Regulation

  • repressed by [protein|search|SinR] [Pubmed|15661000]

    • Regulatory mechanism

  • [protein|search|SinR]: transcription anti-activation (prevents binding of [protein|search|RemA]) [Pubmed|23646920]
  • [protein|search|RemA]: transcription activation [Pubmed|23646920]
  • [protein|search|AbrB]: transcription repression [Pubmed|20817675]

    • Additional information

  • induction by sequestration of [protein|search|SinR] by [protein|search|SinI] or [protein|search|SlrA] [PubMed|15661000,19788541] or by SlrR [PubMed|20351052]
  • the '[protein|search|epsA]-[protein|search|epsB]-[protein|search|epsC]-[protein|search|epsD]-[protein|search|epsE]-[protein|search|epsF]-[protein|search|epsG]-[protein|search|epsH]-[protein|search|epsI]-[protein|search|epsJ]-[protein|search|epsK]-[protein|search|epsL]-[protein|search|epsM]-[protein|search|epsN]-[protein|search|epsO]' operon is not expressed in a '[protein|search|ymdB]' mutant [PubMed|21856853]
  • the amount of the mRNA is substantially decreased upon depletion of [Rny|search|RNase Y] (this is likely due to the increased stability of the '[protein|search|sinR]' mRNA) [PubMed|21815947]

    • Biological materials

    Mutant

  • GP1517 (aphA3) [Pubmed|24493247], available in [SW| Jörg Stülke]'s lab
  • GP1519 (''[protein|search|epsA]-[protein|search|epsB]'', aphA3) [Pubmed|24493247], available in [SW| Jörg Stülke]'s lab
  • GP1567 ''[protein|search|epsA]''::aphA3 ''[protein|search|tkmA]''::spc [Pubmed|24493247], available in [SW| Jörg Stülke]'s lab

    • GFP fusion

  • GP1569 epsA-gfp (spc), available in [SW| Jörg Stülke]'s lab

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]) [Pubmed|24493247], available in [SW| Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1526 epsA-FLAG 3x spc (based on [SW|pGP1331]) [Pubmed|24493247], available in [SW| Jörg Stülke]'s lab

    • Antibody

  • **

    • Labs working on this gene/protein

  • [SW|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]

    • References

    Reviews

  • 20735481

    • Original publications

  • 15661000,16430695,18047568,18647168,20351052,20817675,21856853,21815947,23646920,24493247,25085422,26283769