SubtiBank SubtiBank
epsA
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epsA

extracellular polysaccharide synthesis, putative transmembrane modulator of EpsB activity, might activate EpsB autophosphorylation and substrate phosphorylation
Locus
BSU_34370
Isoelectric point
6.07
Molecular weight
25.75 kDa
Protein length
234 aa Sequence Blast
Gene length
705 bp Sequence Blast
Function
biofilm formation
Product
unknown
Essential
no
Synonyms
yveK

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,529,151 → 3,529,855

The protein

Protein family

  • CapA family (with CapA and TkmA, according to UniProt)
  • CpsC/CapA family (with TkmA, according to UniProt)
  • Paralogous protein(s)

    Localization

  • cell membrane (according to UniProt)
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • RemA: activation, PubMed, in RemA regulon
  • AbrB: repression, PubMed, in AbrB regulon
  • SinR: anti-activation, (prevents binding of RemA) PubMed, in SinR regulon
  • EAR riboswitch: processive antitermination, in EAR riboswitch
  • Regulation

  • repressed by SinR PubMed
  • Additional information

  • induction by sequestration of SinR by SinI or SlrA PubMed
  • expression is increased in rsiX mutants PubMed
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    Biological materials

    Mutant

  • MGNA-A073 (yveK::erm), available at the NBRP B. subtilis, Japan
  • GP1517 (aphA3) PubMed, available in Jörg Stülke's lab
  • GP1519 (epsA-epsB, aphA3) PubMed, available in Jörg Stülke's lab
  • GP1567 epsA::aphA3 tkmA::spc PubMed, available in Jörg Stülke's lab
  • BKE34370 (ΔepsA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTATTCATAGCCTTCAG, downstream forward: _UP4_AAACATTTCGGGGAGTGAAG
  • BKK34370 (ΔepsA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTATTCATAGCCTTCAG, downstream forward: _UP4_AAACATTTCGGGGAGTGAAG
  • GFP fusion

  • GP1569 epsA-gfp (spc), available in Jörg Stülke's lab
  • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH) PubMed, available in Jörg Stülke's lab
  • FLAG-tag construct

  • GP1526 epsA-FLAG 3x spc (based on pGP1331) PubMed, available in Jörg Stülke's lab
  • Antibody

  • **
  • Labs working on this gene/protein

  • Richard Losick, Harvard Univ., Cambridge, USA homepage
  • References

    Reviews

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    Original publications

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