clpQ

two-component ATP-dependent protease

Locus: BSU_16150

Isoelectric point: 6.11

Molecular weight: 19.33 kDa

Protein length: 181 aa

Gene length: 546 bp

Function: protein degradation

Product: two-component ATP-dependent protease

Essential: no

Synonyms: hslV,codW

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Proteolysis → Additional proteins involved in proteolysis

Gene

Coordinates: 1,688,114 → 1,688,659

Phenotypes of a mutant

• impaired in swarming motility PubMed

The protein

Protein family

• peptidase T1B family (single member, according to UniProt)

Structure

• 2Z3B

Localization

• cytoplasm, forms small foci, usually positioned near the cell membrane, formation of foci depends on the presence of the cognate ATPase ClpY PubMed

Expression and Regulation

Operons

• Genes: trmFO-codV-clpQ-clpY-codY
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CodY: repression, PubMed, in CodY regulon

  Regulation

  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

  Additional information

  • the intracellular concentration of CodY is about 2.5 myM (according to PubMed)

Biological materials

Mutant

• BKE16150 (ΔclpQ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAAAGATGACATAAAGGGCC, downstream forward: _UP4_CTTGAATAGAAAGGACTTGA
• BKK16150 (ΔclpQ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAAAGATGACATAAAGGGCC, downstream forward: _UP4_CTTGAATAGAAAGGACTTGA

References

Reviews

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Original publications

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