rhaR

transcriptional regulator ([SW|DeoR family]) of the [gene|C32AE30BF7931D6171E9DE75C67504F81DD0F227|rhaEW]-[gene|F574142C1E61788FF8F4B61280AD4F87292D08DF|rhaR]-[gene|693040A5BA7A25AD485B7B28DBD6E8AAC77450F7|rhaB]-[gene|294C0CC675088B2A2EEFC26FAF1A628002B82C59|rhaM]-[gene|FDDC16D57752D81299CDD426257C1CD49890C89C|rhaA] operon

locus: BSU_31210

pI: 7.37

mw: 28.70 kDa

protein length: 258 aa

gene length: 777 bp

function: control of rhamnose utilization

product: transcriptional regulator ([SW|DeoR family])

essential: no

synonyms: yulB

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.12|Utilization of rhamnose]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 3,201,027 → 3,201,803

The protein

[SW|Domains]

• [SW|HTH deoR-type domain] (aa 3-58) (according to UniProt)

Effectors of protein activity

• molecular inducer: rhamnulose-1-phosphate [Pubmed|26712933]

Expression and Regulation

Operons

• genes: [gene|C32AE30BF7931D6171E9DE75C67504F81DD0F227|rhaEW]-[gene|F574142C1E61788FF8F4B61280AD4F87292D08DF|rhaR]-[gene|693040A5BA7A25AD485B7B28DBD6E8AAC77450F7|rhaB]-[gene|294C0CC675088B2A2EEFC26FAF1A628002B82C59|rhaM]-[gene|FDDC16D57752D81299CDD426257C1CD49890C89C|rhaA]
  description: [Pubmed|26712933,22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|26712933], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|F574142C1E61788FF8F4B61280AD4F87292D08DF|RhaR]: repression, [Pubmed|26712933], in [regulon|F574142C1E61788FF8F4B61280AD4F87292D08DF|RhaR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|26712933], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • expression during spore [SW|germination] is increased under conditions of osmotic stress [Pubmed|27766092]

• genes: [gene|F574142C1E61788FF8F4B61280AD4F87292D08DF|rhaR]-[gene|693040A5BA7A25AD485B7B28DBD6E8AAC77450F7|rhaB]-[gene|294C0CC675088B2A2EEFC26FAF1A628002B82C59|rhaM]-[gene|FDDC16D57752D81299CDD426257C1CD49890C89C|rhaA]
  description: [Pubmed|22383849]

  regulation

  • expression during spore [SW|germination] is increased under conditions of osmotic stress [Pubmed|27766092]

Biological materials

Mutant

• MGNA-B546 (yulB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1545 NBRP B. subtilis, Japan]
• BKE31210 (Δ[gene|F574142C1E61788FF8F4B61280AD4F87292D08DF|rhaR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGGTAACGCCCTTCCTC, downstream forward: _UP4_CCTCTTTCGAAGAGAGGGTG
• BKK31210 (Δ[gene|F574142C1E61788FF8F4B61280AD4F87292D08DF|rhaR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGGTAACGCCCTTCCTC, downstream forward: _UP4_CCTCTTTCGAAGAGAGGGTG

References

• 26712933,24391637,22383849