rhaR

transcriptional regulator (DeoR family) of the rhaEW-rhaR-rhaB-rhaM-rhaA operon

Locus: BSU_31210

Isoelectric point: 7.37

Molecular weight: 28.70 kDa

Protein length: 258 aa

Gene length: 777 bp

Function: control of rhamnose utilization

Product: transcriptional regulator (DeoR family)

Essential: no

Synonyms: yulB

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of rhamnose
• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other

Gene

Coordinates: 3,201,027 → 3,201,803

The protein

Domains

• HTH deoR-type domain (aa 3-58) (according to UniProt)

Effectors of protein activity

• molecular inducer: rhamnulose-1-phosphate PubMed

Expression and Regulation

Operons

• Genes: rhaEW-rhaR-rhaB-rhaM-rhaA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • RhaR: repression, PubMed, in RhaR regulon
  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • expression during spore germination is increased under conditions of osmotic stress PubMed

• Genes: rhaR-rhaB-rhaM-rhaA
  Description: PubMed

  Regulation

  • expression during spore germination is increased under conditions of osmotic stress PubMed

Biological materials

Mutant

• MGNA-B546 (yulB::erm), available at the NBRP B. subtilis, Japan
• BKE31210 (ΔrhaR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGGTAACGCCCTTCCTC, downstream forward: _UP4_CCTCTTTCGAAGAGAGGGTG
• BKK31210 (ΔrhaR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGGTAACGCCCTTCCTC, downstream forward: _UP4_CCTCTTTCGAAGAGAGGGTG

References

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