SubtiBank SubtiBank
queE [2019-01-30 12:37:00]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

queE [2019-01-30 12:37:00]

7-carboxy-7-deazaguanine (CDG) synthase, required for the synthesis of the modified ribonucleotide queuosine
locus
BSU13740
pI
4.96
mw
26.97 kDa
protein length
243 aa Sequence Blast
gene length
732 bp Sequence Blast
function
tRNA modification
product
7-carboxy-7-deazaguanine (CDG) synthase
essential
no
synonyms
ykvL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]

    • Gene

    Coordinates
    1,440,542 → 1,441,273

    The protein

    Catalyzed reaction/ biological activity

  • radical-mediated ring rearrangement required to convert 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) into 7-carboxy-7-deazaguanine (CDG) [pubmed|29303575]

    • Protein family

  • radical S-adenosyl-L-methionine (SAM) superfamily

    • [SW|Cofactors]

  • Fe-S cluster [pubmed|29292548]
  • S-adenosyl-L-methionine, Mg(2+) [Pubmed|23194065]

    • Structure

  • [PDB|5TGS] [pubmed|28045519]

    • Expression and Regulation

    Operons

    genes
    [gene|1925DFD1162E744E9175D07EC0FD72A75C79C6BF|queC]-[gene|BA6DD5C9AB9BFF9759E9ECC46CE3DF4EB7E67F52|queD]-[gene|F5189CAA5EF43FCF95E593E095F84B3BE486B347|queE]-[gene|5F96500CC46CC4DAECEBAD34EC50B10548E6D0F9|queF]
    description
    [Pubmed|14660578]

    regulatory mechanism

  • [regulon|preQ1 riboswitch|preQ1 riboswitch]: antitermination, in the absence of queuosine [Pubmed|19285444], in [regulon|preQ1 riboswitch|preQ1 riboswitch]

    • regulation

  • repressed in the presence of queuosine ([SW|preQ1 riboswitch]) [Pubmed|19285444]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A790 (ykvL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/790 NBRP B. subtilis, Japan]
  • BKE13740 (Δ[gene|F5189CAA5EF43FCF95E593E095F84B3BE486B347|queE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAAAATTTCTAATACAGGAA, downstream forward: _UP4_GTATAATAGAAAGGAAGATG
  • BKK13740 (Δ[gene|F5189CAA5EF43FCF95E593E095F84B3BE486B347|queE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAAAATTTCTAATACAGGAA, downstream forward: _UP4_GTATAATAGAAAGGAAGATG

    • References

  • 14660578,19285444,19354300,17384645,24362703,23194065,25933252,29303575,28045519