yodU [2017-05-24 08:37:25]

N-terminal part of the split gene spsM (together with ypqP), spore envelope polysaccharide biosynthesis, in B. subtilis 168 the gene is disrupted by the SP-beta prophage

locus: BSU19810

pI: 9.82

mw: 15.00 kDa

protein length: 141 aa

gene length: 423 bp

function: spore envelope polysaccharide biosynthesis

product: unknown

essential: no

synonyms: ypqP, spsM

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.8|Biosynthesis of legionaminic acid (for spore crust))]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]

Gene

Coordinates: 2,151,626 → 2,152,045

The protein

Paralogous protein(s)

• [protein|5DB168A3D087AAEB003D7548A1A4356ABD07F712|EpsC] (for SpsM)

[SW|Domains]

• SpsM: Polysacc_synt_2 domain (Pfam accession number, PF02719) in the 18–296-aa region [Pubmed|25299644]

[SW|Localization]

• secreted (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|F42B27D5D7F3F39828940E377ABCC0730B25EA88|yodU]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|25299644,15383836], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

  regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: activation, [Pubmed|25299644,15383836], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]

  regulation

  • expressed late during [SW|sporulation] in the mother cell ([protein|search|SigK], [protein|search|GerE]) [Pubmed|25299644,15383836]

Biological materials

Mutant

• MGNA-A060 (yodU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/60 NBRP B. subtilis, Japan]

References

• 25299644,15383836,25326298