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yodU [2020-09-09 15:58:05]
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yodU [2020-09-09 15:58:05]

5' part of the split gene ypqP (together with ypqP), legionaminic acid synthesis and addition on the spore surface, in B. subtilis 168 the gene is disrupted by the SP-beta prophage
Locus
BSU_19810
Isoelectric point
9.82
Molecular weight
15.00 kDa
Protein length
139 aa Sequence Blast
Gene length
420 bp Sequence Blast
Function
legionaminic acid synthesis and addition on the spore surface
Product
UDP-NAcGlcA inverting 4,6-dehydratase (N-terminal part)
Essential
no
Synonyms
ypqP,spsM

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,151,626 → 2,152,045

Phenotypes of a mutant

  • the ypqP mutant of PY79 has a less hydrophobic spore surface PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • required for spore crust assembly, legionaminic acid synthesis and addition on the spore surface PubMed
  • Paralogous protein(s)

    Domains

  • SpsM: Polysacc_synt_2 domain (Pfam accession number, PF02719) in the 18–296-aa region PubMed
  • Structure

  • 2GN4 (from Helicobacter pylori, 46% identity) PubMed
  • Localization

  • secreted (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigK: sigma factor, PubMed, in SigK regulon
  • Regulatory mechanism

  • GerE: activation, PubMed, in GerE regulon
  • Regulation

  • expressed late during sporulation in the mother cell (SigK, GerE) PubMed
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    Biological materials

    Mutant

  • MGNA-A060 (yodU::erm), available at the NBRP B. subtilis, Japan
  • BKE19810 (ΔyodU::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGCTTATCCTCCAACTC, downstream forward: _UP4_GTATATTAAGATACTTACTA
  • BKK19810 (ΔyodU::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGCTTATCCTCCAACTC, downstream forward: _UP4_GTATATTAAGATACTTACTA
  • References

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