phrI

response regulator aspartate phosphatase (RapI) regulator

Locus: BSU_05020

Isoelectric point: 8.91

Molecular weight: 4.10 kDa

Protein length: 39 aa

Gene length: 120 bp

Function: control of the transfer of the mobile genetic element ICEBs1

Product: phosphatase (RapI) regulator

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → Transcription factors and their control → Control of transcription factor (other than two-component system)
• Information processing → Regulation of gene expression → Quorum sensing
• Prophages and mobile genetic elements → Mobile genetic elements → ICEBs1
• Groups of genes → Short peptides

Gene

Coordinates: 548,438 → 548,557

The protein

Catalyzed reaction/ biological activity

• binds RapI and inhibits its activity, this results in contiuned repression of the genes for the transfer of the mobile genetic element ICEBs1 by ImmR PubMed

Protein family

• phr family (according to UniProt)

Expression and Regulation

Operons

• Genes: rapI-phrI
  Description: PubMed

  Sigma factors

  • SigH: sigma factor, PubMed, in SigH regulon
  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • ComA: activation, PubMed, in ComA regulon

  Regulation

  • induced at high cell density (ComA) PubMed

• Genes: phrI
  Description: PubMed

  Regulation

  • induced at high cell density (ComA) PubMed

Biological materials

Mutant

• BKE05020 (ΔphrI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTACTTAAAATCACTGCT, downstream forward: _UP4_TAGCTTAGATAATTGGAAAA
• BKK05020 (ΔphrI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTACTTAAAATCACTGCT, downstream forward: _UP4_TAGCTTAGATAATTGGAAAA

References

Reviews

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Original publications

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