ylaM [2017-8-11 16:0:3]

glutaminase, high affinity for glutamine

locus: BSU14830

pI: 5.68

mw: 33.86 kDa

protein length: 309 aa

gene length: 927 bp

function: glutamine degradation

product: glutaminase

essential: no

ec: 3.5.1.2

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.1|Utilization of glutamine/ glutamate]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]

Gene

Coordinates: 1,551,385 → 1,552,314

The protein

Catalyzed reaction/ biological activity

• L-glutamine + H₂O = L-glutamate + NH₃ (according to Swiss-Prot)

Protein family

• glutaminase family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|1A415DC85354373EA4866733C3AE43F510BD3C56|GlsA]

Structure

• [PDB|3BRM] (the structure of [protein|1A415DC85354373EA4866733C3AE43F510BD3C56|GlsA]) [Pubmed|18459799]

Expression and Regulation

Operons

• genes: [gene|5913ABC121A03644897BD365D7E507661CD3AF39|ylaK]-[gene|F31DD310F0D80ECC7C6D1E5DDB8EAF32565A3D51|ylaM]
  description: [pubmed|22383849]
  regulation: expressed during [SW|sporulation] [pubmed|22383849]

Biological materials

Mutant

• MGNA-A542 (ylaM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/542 NBRP B. subtilis, Japan]

References

• 18459799