ylaM

glutaminase, high affinity for glutamine

locus: BSU_14830

pI: 5.68

mw: 33.86 kDa

protein length: 309 aa

gene length: 930 bp

function: glutamine degradation

product: glutaminase

essential: no

ec: 3.5.1.2

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.1|Utilization of glutamine/ glutamate]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]

Gene

Coordinates: 1,551,385 → 1,552,314

The protein

Catalyzed reaction/ biological activity

• H₂O + L-glutamine --> L-glutamate + NH₄⁺ (according to UniProt)

Protein family

• glutaminase family (with [protein|F31DD310F0D80ECC7C6D1E5DDB8EAF32565A3D51|YlaM], according to UniProt)

Paralogous protein(s)

• [protein|1A415DC85354373EA4866733C3AE43F510BD3C56|GlsA]

Structure

• [PDB|3BRM] (the structure of [protein|1A415DC85354373EA4866733C3AE43F510BD3C56|GlsA]) [Pubmed|18459799]

Expression and Regulation

Operons

• genes: [gene|5913ABC121A03644897BD365D7E507661CD3AF39|ylaK]-[gene|F31DD310F0D80ECC7C6D1E5DDB8EAF32565A3D51|ylaM]
  description: [pubmed|22383849]
  regulation: expressed during [SW|sporulation] [pubmed|22383849]

Biological materials

Mutant

• MGNA-A542 (ylaM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/542 NBRP B. subtilis, Japan]
• BKE14830 (Δ[gene|F31DD310F0D80ECC7C6D1E5DDB8EAF32565A3D51|ylaM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCAAACACCCTCGTTCC, downstream forward: _UP4_TAATTTATGTCATATGCTTA
• BKK14830 (Δ[gene|F31DD310F0D80ECC7C6D1E5DDB8EAF32565A3D51|ylaM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCAAACACCCTCGTTCC, downstream forward: _UP4_TAATTTATGTCATATGCTTA

Expression vector

• pBP768: IPTG inducible expression, purification in E. coli with cleaveable N-terminal His-tag, in petSUMOadapt, available in [SW|Fabian Commichau]'s and [SW|Jörg Stülke]'s labs

References

• 18459799