skfH

unknown

Locus: BSU_01980

Isoelectric point: 4.82

Molecular weight: 16.08 kDa

Protein length: 141 aa

Gene length: 426 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ybdE

Genomic Context

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Categories containing this gene/protein

• Prophages and mobile genetic elements → Prophages → Prophage 1
• Groups of genes → Proteins of unknown function

Gene

Coordinates: 219,607 → 220,032

The protein

Domains

• Thioredoxin domain (aa 2-141) (according to UniProt)

Structure

• 2HYX (from Mycobacterium tuberculosis, 33% identity)

Expression and Regulation

Operons

• Genes: skfA-skfB-skfC-skfE-skfF-skfG-skfH
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • Spo0A: activation, PubMed, in Spo0A regulon
  • AbrB: repression, PubMed, in AbrB regulon
  • PhoP: activation, PubMed, in PhoP regulon

  Regulation

  • expressed under conditions that trigger sporulation (Spo0A) PubMed

  Additional information

  • Northern blotting during during phosphate limitation showed an intense 0.25 kb 'SkfA'-specific transcript, and a weaker 6.5 kb skfA-skfB-skfC-skfE-skfF-skfG-skfH transcript

Biological materials

Mutant

• MGNA-B954 (ybdE::erm), available at the NBRP B. subtilis, Japan
• BKE01980 (ΔskfH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCTTTCAGTCCTCCTTA, downstream forward: _UP4_TGAAATTTTCCGTCTTGTAT
• BKK01980 (ΔskfH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCTTTCAGTCCTCCTTA, downstream forward: _UP4_TGAAATTTTCCGTCTTGTAT

References

Reviews

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Original Publications

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