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ywbD [2019-01-21 10:10:01]
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ywbD [2019-01-21 10:10:01]

similar to ribosomal RNA large subunit methyltransferase I
Locus
BSU38360
Isoelectric point
9.18
Molecular weight
44.28 kDa
Protein length
396 aa Sequence Blast
Gene length
1188 bp Sequence Blast
Function
unknown
Product
putative ribosomal RNA large subunit methyltransferase
Essential
no
Synonyms
ipa-19d
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → rRNA modification and maturation/ based on similarity

    • Gene

    Coordinates
    3,935,824 → 3,937,014

    The protein

    Catalyzed reaction/ biological activity

  • S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing C(5)-methylcytosine (according to Swiss-Prot)

    • Protein family

  • RlmI family (according to Swiss-Prot)

    • Localization

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    Genes
    ywbD
    Description
    PubMed

    Regulatory mechanism

  • SinR: repression, PubMed, in SinR regulon
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    Biological materials

    Mutant

  • MGNA-B222 (ywbD::erm), available at the NBRP B. subtilis, Japan
  • BKE38360 (ΔywbD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATATGTCGTCCTCTTTC, downstream forward: _UP4_TAAATGAAAAAGCTGCCCTG
  • BKK38360 (ΔywbD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATATGTCGTCCTCTTTC, downstream forward: _UP4_TAAATGAAAAAGCTGCCCTG

    • LacZ fusion

  • GP1614 amyE::p(ywbD-lacZ cat), constructed with pGP2150 based on pAC5, available in Jörg Stülke's lab

    • References

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