ywbD

similar to ribosomal RNA large subunit methyltransferase I

locus: BSU_38360

pI: 9.18

mw: 44.28 kDa

protein length: 396 aa

gene length: 1191 bp

function: unknown

product: putative ribosomal RNA large subunit methyltransferase

essential: no

synonyms: ipa-19d

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.3|rRNA modification and maturation/ based on similarity]

Gene

Coordinates: 3,935,824 → 3,937,014

The protein

Protein family

• [SW|Methyltransferase superfamily] (according to UniProt)

[SW|Domains]

• PUA domain (aa 1-79) (according to UniProt)

Structure

• [PDB|3VSE] (from Staphylococcus aureus, 48% identity) [pubmed|23016631]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|F2F0B16E5F55C8C91C20E4FFC7A63E3CD09D4EFD|ywbD]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, [Pubmed|16430695], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]

Biological materials

Mutant

• MGNA-B222 (ywbD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1221 NBRP B. subtilis, Japan]
• BKE38360 (Δ[gene|F2F0B16E5F55C8C91C20E4FFC7A63E3CD09D4EFD|ywbD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATGTCGTCCTCTTTC, downstream forward: _UP4_TAAATGAAAAAGCTGCCCTG
• BKK38360 (Δ[gene|F2F0B16E5F55C8C91C20E4FFC7A63E3CD09D4EFD|ywbD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATGTCGTCCTCTTTC, downstream forward: _UP4_TAAATGAAAAAGCTGCCCTG

lacZ fusion

• GP1614 ''amyE::p(ywbD-lacZ cat)'', constructed with pGP2150 based on [SW|pAC5], available in [SW| Jörg Stülke]'s lab

References

• 16430695,18786544,23016631