lipL

N-amidotransferase, transfers lipoic acid from lipoyl-[protein|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|GcvH] to [protein|C4B6C3EE560C8BD353FEABB1A607C89C20DC8D34|AcoC], [protein|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|BkdB], [protein|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|OdhB], and [protein|2F40086E35FA32136B9A89C530A86D714FE9460C|PdhC], and from lipoyl-[protein|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|OdhB] to [protein|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|BkdB] and [protein|2F40086E35FA32136B9A89C530A86D714FE9460C|PdhC]

locus: BSU_37640

pI: 5.27

mw: 31.27 kDa

protein length: 281 aa

gene length: 846 bp

function: lipoic acid metabolism

product: protein N-octanoyltransferase

essential: no

ec: 2.3.1.204

synonyms: ipa-90d,ywfL

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.7|Biosynthesis and scavenging of lipoic acid]

Gene

Coordinates: 3,863,415 → 3,864,260

The protein

Catalyzed reaction/ biological activity

• transfers lipoic acid from lipoyl-[protein|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|GcvH] to [protein|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|BkdB], [protein|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|OdhB], and [protein|2F40086E35FA32136B9A89C530A86D714FE9460C|PdhC], and from lipoyl-[protein|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|OdhB] to [protein|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|BkdB] and [protein|2F40086E35FA32136B9A89C530A86D714FE9460C|PdhC] [pubmed|31066113]
• [glycine-cleavage complex H protein]-N⁶-octanoyl-L-lysine + [lipoyl-carrier protein]-L-lysine --> [glycine-cleavage complex H protein]-L-lysine + [lipoyl-carrier protein]-N⁶-octanoyl-L-lysine (according to UniProt)

Protein family

• Octanoyltransferase LipL family (single member, according to UniProt)

[SW|Domains]

• [SW|BPL/LPL catalytic domain] (aa 45-253) (according to UniProt)

Structure

• [PDB|2P5I] (from ''Bacillus halodurans'', 56% identity)

Expression and Regulation

Operons

• genes: [gene|F2E881F4BE0968C75BD75A5EFF4C7CA642034796|lipL]
  description: [Pubmed|9353933]

Biological materials

Mutant

• MGNA-B667 (ywfL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1666 NBRP B. subtilis, Japan]
• BKE37640 (Δ[gene|F2E881F4BE0968C75BD75A5EFF4C7CA642034796|lipL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATGTAAAACCCTTTC, downstream forward: _UP4_TGAACGTTCTTCGCATGCCG
• BKK37640 (Δ[gene|F2E881F4BE0968C75BD75A5EFF4C7CA642034796|lipL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATGTAAAACCCTTTC, downstream forward: _UP4_TGAACGTTCTTCGCATGCCG

References

Reviews

• 27074917

Original publications

• 9353933,21338420,21338421,31066113