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ywqL [2019-08-12 09:33:03]
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ywqL [2019-08-12 09:33:03]

endonuclease V
locus
BSU_36170
pI
5.71
mw
26.83 kDa
protein length
238 aa Sequence Blast
gene length
717 bp Sequence Blast
function
DNA repair
product
endonuclease V
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]

    • Gene

    Coordinates
    3,724,420 → 3,725,136

    Phenotypes of a mutant

  • increased mutation rates [Pubmed|22056936]
  • increased sensitivity of sporulating cells to the genotoxic effects of nitrous acid[Pubmed|27698084]
  • increased mutation rates [pubmed|30726292]

    • The protein

    Catalyzed reaction/ biological activity

  • Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate (according to Swiss-Prot)
  • in vitro endonuclease activity against double-stranded DNAs containing a single uracil (U), hypoxanthine (Hx), xanthine (X) or an AP site [pubmed|30726292]
  • catalyzes a single strand break at the second phosphodiester bond towards the 3'-end of the U and AP lesions [pubmed|30726292]

    • Protein family

  • endonuclease V family (single member, according to UniProt)

    • Structure

  • [PDB|3GA2]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|2401A4E3E5E683472C51AB2F31BC4F54F672F32A|ywqH]-[gene|04376B3166B591132CE37D1DE85B0ECFEC6902EF|ywqI]-[gene|2F9C623DDF9F93EFD46F024FDA3AF2A92FC9BF7A|ywqJ]-[gene|7ED407717C216FCB2B281A3ECFAA2ED594F021AF|ywqK]-[gene|F2C6956D69FBE57933E46136436E8E80B63DF8BB|ywqL]
    description
    [Pubmed|22383849]

    regulation

  • expressed throughout growth and staionary phase [Pubmed|22056936]
    • view in new tab

    genes
    [gene|F2C6956D69FBE57933E46136436E8E80B63DF8BB|ywqL]
    description
    [Pubmed|9353933]

    regulation

  • expressed throughout growth and staionary phase [Pubmed|22056936]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A566 (ywqL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/566 NBRP B. subtilis, Japan]
  • BKE36170 (Δ[gene|F2C6956D69FBE57933E46136436E8E80B63DF8BB|ywqL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCAAATACCTTCATAACGA, downstream forward: _UP4_TAACACTTGGGGCTCTAAGT
  • BKK36170 (Δ[gene|F2C6956D69FBE57933E46136436E8E80B63DF8BB|ywqL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCAAATACCTTCATAACGA, downstream forward: _UP4_TAACACTTGGGGCTCTAAGT

    • References

  • 9353933,22056936,22383849,27530626,27698084,30726292