ywqL

endonuclease V

locus: BSU_36170

pI: 5.71

mw: 26.83 kDa

protein length: 238 aa

gene length: 717 bp

function: DNA repair

product: endonuclease V

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]

Gene

Coordinates: 3,724,420 → 3,725,136

Phenotypes of a mutant

• increased mutation rates [Pubmed|22056936]
• increased sensitivity of sporulating cells to the genotoxic effects of nitrous acid[Pubmed|27698084]
• increased mutation rates [pubmed|30726292]

The protein

Catalyzed reaction/ biological activity

• Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate (according to UniProt)
• in vitro endonuclease activity against double-stranded DNAs containing a single uracil (U), hypoxanthine (Hx), xanthine (X) or an AP site [pubmed|30726292]
• catalyzes a single strand break at the second phosphodiester bond towards the 3'-end of the U and AP lesions [pubmed|30726292]

Protein family

• endonuclease V family (single member, according to UniProt)

Structure

• [PDB|3GA2]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|2401A4E3E5E683472C51AB2F31BC4F54F672F32A|ywqH]-[gene|04376B3166B591132CE37D1DE85B0ECFEC6902EF|ywqI]-[gene|2F9C623DDF9F93EFD46F024FDA3AF2A92FC9BF7A|ywqJ]-[gene|7ED407717C216FCB2B281A3ECFAA2ED594F021AF|ywqK]-[gene|F2C6956D69FBE57933E46136436E8E80B63DF8BB|ywqL]
  description: [Pubmed|22383849]

  regulation

  • expressed throughout growth and staionary phase [Pubmed|22056936]

• genes: [gene|F2C6956D69FBE57933E46136436E8E80B63DF8BB|ywqL]
  description: [Pubmed|9353933]

  regulation

  • expressed throughout growth and staionary phase [Pubmed|22056936]

Biological materials

Mutant

• MGNA-A566 (ywqL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/566 NBRP B. subtilis, Japan]
• BKE36170 (Δ[gene|F2C6956D69FBE57933E46136436E8E80B63DF8BB|ywqL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCAAATACCTTCATAACGA, downstream forward: _UP4_TAACACTTGGGGCTCTAAGT
• BKK36170 (Δ[gene|F2C6956D69FBE57933E46136436E8E80B63DF8BB|ywqL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCAAATACCTTCATAACGA, downstream forward: _UP4_TAACACTTGGGGCTCTAAGT

References

• 9353933,22056936,22383849,27530626,27698084,30726292