manR

transcriptional activator of the [gene|575CEC5C5C0458DC86A74F24654AC6990CB1E732|manP]-[gene|E26C70893C5D677C816C814558CC42F90B920087|manA]-[gene|2DB0F763349D34FB1FCBBB4665416E129AD0AE6A|yjdF] operon

locus: BSU_12000

pI: 8.04

mw: 75.13 kDa

protein length: 648 aa

gene length: 1947 bp

function: regulation of mannose utilization

product: transcriptional activator, PRD-type

essential: no

synonyms: yjdC

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.9|Utilization of mannose]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.3|PRD-type regulators]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

Gene

Coordinates: 1,270,631 → 1,272,577

The protein

Catalyzed reaction/ biological activity

• D-mannose + N^π-phospho-L-histidyl-[protein] --> D-mannose 6-phosphate + L-histidyl-[protein] (according to UniProt)

Protein family

• [SW|PRD-containing transcription factors]

Modification

• phosphorylation by [protein|575CEC5C5C0458DC86A74F24654AC6990CB1E732|ManP] inactivates [protein|F273002AF97D87BB025B4F014C328C5592EAD621|ManR] (in the absence of mannose), phosphorylation by [gene|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr] stimluates [protein|F273002AF97D87BB025B4F014C328C5592EAD621|ManR] [Pubmed|20139185]

Expression and Regulation

Operons

• genes: [gene|F273002AF97D87BB025B4F014C328C5592EAD621|manR]
  description: [Pubmed|20139185]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|20139185], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|F273002AF97D87BB025B4F014C328C5592EAD621|ManR]: activation, in the presence of mannose and absence of glucose [Pubmed|20139185], in [regulon|F273002AF97D87BB025B4F014C328C5592EAD621|ManR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|22900538], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • induced by mannose ([protein|search|ManR]) [Pubmed|20139185]

Biological materials

Mutant

• MGNA-A269 (yjdC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/269 NBRP B. subtilis, Japan]
• BKE12000 (Δ[gene|F273002AF97D87BB025B4F014C328C5592EAD621|manR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTTTTTCTATCCTTC, downstream forward: _UP4_TAAAAGCAGGGATTATTCCT
• BKK12000 (Δ[gene|F273002AF97D87BB025B4F014C328C5592EAD621|manR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTTTTTCTATCCTTC, downstream forward: _UP4_TAAAAGCAGGGATTATTCCT

References

• 10627040,20139185,9663674,22900538,23551403