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ywrD [2019-02-27 17:30:13]
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ywrD [2019-02-27 17:30:13]

unknown, similar to gamma-glutamyltransferase (Ggt)
locus
BSU36100
pI
5.45
mw
57.32 kDa
protein length
525 aa Sequence Blast
gene length
1578 bp Sequence Blast
function
unknown
product
unknown
essential
no
ec
2.3.2.2
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

    • Gene

    Coordinates
    3,719,134 → 3,720,711

    The protein

    Catalyzed reaction/ biological activity

  • (5-L-glutamyl)-peptide + an amino acid = peptide + 5-L-glutamyl amino acid (according to Swiss-Prot), however, all evidence suggests this is a misannotation [Pubmed|19935659]

    • Protein family

  • gamma-glutamyltransferase family (according to Swiss-Prot), however, all evidence suggests this is a misannotation [Pubmed|19935659]

    • Additional information

  • The gene is annotated in KEGG as an ortholog of gamma-glutamyltranspeptidase EC 2.3.2.2, This enzyme is necessary to utilize glutathione (GSH) as the sulfur source. No EC annotation for this gene is available in Swiss-Prot/MetaCyc. It has been shown by Minami et al. ([Pubmed|14762019]) that ywrD mutant grows well on minimal media supplied with GSH as the sole sulfur source. In addition, His-tag purified ywrD cannot hydrolyze GSH. [Pubmed|19935659]

    • Expression and Regulation

    Operons

    genes
    [gene|F1DF59528E340A65A982BD7E6621DDA82C10B2B5|ywrD]
    description
    [Pubmed|12823818]

    regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, [Pubmed|12823818], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]

    • regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]
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    Biological materials

    Mutant

  • MGNA-B653 (ywrD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1652 NBRP B. subtilis, Japan]
  • BKE36100 (Δ[gene|F1DF59528E340A65A982BD7E6621DDA82C10B2B5|ywrD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACAAGTCCCCTTTTTT, downstream forward: _UP4_GGAGCGGCTGTGGGGATTTA
  • BKK36100 (Δ[gene|F1DF59528E340A65A982BD7E6621DDA82C10B2B5|ywrD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACAAGTCCCCTTTTTT, downstream forward: _UP4_GGAGCGGCTGTGGGGATTTA

    • References

  • 12823818,25755103,14762019