ywrD

unknown, similar to gamma-glutamyltransferase (Ggt)

Locus: BSU_36100

Isoelectric point: 5.45

Molecular weight: 57.32 kDa

Protein length: 525 aa

Gene length: 1578 bp

Function: unknown

Product: unknown

Essential: no

E.C.: 2.3.2.2

Synonyms

Genomic Context

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Categories containing this gene/protein

• Groups of genes → Proteins of unknown function

Gene

Coordinates: 3,719,134 → 3,720,711

The protein

Catalyzed reaction/ biological activity

• an α-amino acid + an N-terminal (5-L-glutamyl)-[peptide] --> 5-L-glutamyl amino acid + N-terminal L-α-aminoacyl-[peptide] (according to UniProt)
• glutathione + H₂O --> L-cysteinylglycine + L-glutamate (according to UniProt)
• an S-substituted glutathione + H₂O --> an S-substitued L-cysteinylglycine + L-glutamate, however, all evidence suggests this is a misannotation PubMed

Protein family

• gamma-glutamyltransferase family (with Ggt, according to UniProt), however, all evidence suggests this is a misannotation PubMed

Structure

• 2NLZ (from B. halodurans, 38% identity)

Additional information

• The gene is annotated in KEGG as an ortholog of gamma-glutamyltranspeptidase EC 2.3.2.2, This enzyme is necessary to utilize glutathione (GSH) as the sulfur source. No EC annotation for this gene is available in Swiss-Prot/MetaCyc. It has been shown by Minami et al. (PubMed) that ywrD mutant grows well on minimal media supplied with GSH as the sole sulfur source. In addition, His-tag purified ywrD cannot hydrolyze GSH. PubMed

Expression and Regulation

Operons

• Genes: ywrD
  Description: PubMed

  Regulatory mechanism

  • TnrA: activation, PubMed, in TnrA regulon

  Regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

Biological materials

Mutant

• MGNA-B653 (ywrD::erm), available at the NBRP B. subtilis, Japan
• BKE36100 (ΔywrD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCACAAGTCCCCTTTTTT, downstream forward: _UP4_GGAGCGGCTGTGGGGATTTA
• BKK36100 (ΔywrD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCACAAGTCCCCTTTTTT, downstream forward: _UP4_GGAGCGGCTGTGGGGATTTA

References

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