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yitF [2019-06-19 11:17:37]
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yitF [2019-06-19 11:17:37]

similar to mandelate racemase
locus
BSU10970
pI
7.53
mw
41.89 kDa
protein length
371 aa Sequence Blast
gene length
1116 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]
  • Gene

    Coordinates
    1,174,861 → 1,175,976

    The protein

    Protein family

  • mandelate racemase/muconate lactonizing enzyme family (according to Swiss-Prot)
  • Structure

  • [PDB|2GDQ]
  • Expression and Regulation

    Operons

    genes
    [gene|BA1A7ECAE0B5690F6E6A67785BAB18628701E2A2|yitG]-[gene|F1A84EC28BE0E306356A331FD362E3CDAE49A3AB|yitF]
    description
    [Pubmed|16497325]

    sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|16497325,15699190], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]
  • regulation

  • expressed during sporulation in the forespore ([protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]) [Pubmed|16497325,15699190]
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    Biological materials

    Mutant

  • MGNA-B180 (yitF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1179 NBRP B. subtilis, Japan]
  • BKE10970 (Δ[gene|F1A84EC28BE0E306356A331FD362E3CDAE49A3AB|yitF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCGTACAATTTTCACACTGG, downstream forward: _UP4_TAAGCTGGGCCATTTCTTTA
  • BKK10970 (Δ[gene|F1A84EC28BE0E306356A331FD362E3CDAE49A3AB|yitF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCGTACAATTTTCACACTGG, downstream forward: _UP4_TAAGCTGGGCCATTTCTTTA
  • References

  • 16497325,15699190