mccA

O-acetylserine-thiol-lyase

Locus: BSU_27260

Isoelectric point: 5.23

Molecular weight: 32.86 kDa

Protein length: 307 aa

Gene length: 921 bp

Function: methionine-to-cysteine conversion

Product: O-acetylserine-thiol-lyase

Essential: no

Synonyms: yrhA

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of cysteine

Gene

Coordinates: 2,786,142 → 2,787,065

Phenotypes of a mutant

• a mccA cysK double mutant is auxotrophic for cysteine PubMed

The protein

Catalyzed reaction/ biological activity

• L-homocysteine + O-acetyl-L-serine --> acetate + H⁺ + L,L-cystathionine (according to UniProt)

Protein family

• Cysteine synthase/cystathionine beta-synthase family (with CysK and YtkP, according to UniProt)

Paralogous protein(s)

• CysK, YtkP

Cofactors

• PLP (according to UniProt)

Structure

• 4QL4 (from B. anthracis, 63% identity)

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: yrrT-mtnN-mccA-mccB-yrhC
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CymR: repression, PubMed, in CymR regulon
  • Spx: activation, PubMed, in Spx regulon

  Regulation

  • repressed in the presence of cysteine (CymR) PubMed

Biological materials

Mutant

• MGNA-A849 (yrhA::erm), available at the NBRP B. subtilis, Japan
• 1A945 ( mccA::kan), PubMed, available at BGSC
• BKE27260 (ΔmccA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATATGTCCTCCTCCTTA, downstream forward: _UP4_CAAATATACGAAGGAGGCAT
• BKK27260 (ΔmccA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATATGTCCTCCTCCTTA, downstream forward: _UP4_CAAATATACGAAGGAGGCAT

References

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