polY2

translesion synthesis (TLS-) DNA polymerase Y2

Locus: BSU_23710

Isoelectric point: 9.53

Molecular weight: 45.78 kDa

Protein length: 412 aa

Gene length: 1239 bp

Function: UV-targeted mutagenesis

Product: translesion synthesis (TLS-) DNA polymerase Y2

Essential: no

Synonyms: yqjW

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA repair/ recombination
• Information processing → Genetics → DNA repair/ recombination → Other proteins

Gene

Coordinates: 2,464,562 → 2,465,800

Phenotypes of a mutant

• sensitive to blue light-induced DNA damage PubMed
• increased sensitivity to Cr(VI) PubMed

The protein

Catalyzed reaction/ biological activity

• 2'-deoxyribonucleoside 5'-triphosphate + DNA_(n) --> diphosphate + DNA_(n+1) (according to UniProt)
• required for Cr(VI)-induced mutagenesis PubMed

Protein family

• DNA polymerase type-Y family (according to UniProt)

Paralogous protein(s)

• PolY1, UvrX

Domains

• UmuC domain (aa 7-192) (according to UniProt)

Structure

• 4DEZ (from Mycobacterium smegmatis, 30% identity) PubMed

Expression and Regulation

Operons

• Genes: polY2-yqjX-yqjY-yqjZ-yqkA-yqkB-yqkC
  Description: PubMed

  Regulatory mechanism

  • LexA: repression, PubMed, in LexA regulon

  Regulation

  • induced upon DNA damage (LexA) PubMed

Biological materials

Mutant

• MGNA-C403 (yqjW::erm), available at the NBRP B. subtilis, Japan
• GP1505 (polY2::kan), available in Stülke lab PubMed
• BKE23710 (ΔpolY2::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACACACCTCTAAAAAGA, downstream forward: _UP4_GCTAAAATAGGGGGGCATTA
• BKK23710 (ΔpolY2::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACACACCTCTAAAAAGA, downstream forward: _UP4_GCTAAAATAGGGGGGCATTA

References

Reviews

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Original publications

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