yesN

two-component response regulator

Locus: BSU_06960

Isoelectric point: 6.95

Molecular weight: 40.50 kDa

Protein length: 368 aa

Gene length: 1107 bp

Function: unknown

Product: two-component response regulator

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → Transcription factors and their control → Two-component system response regulators
• Groups of genes → Efp-dependent proteins
• Groups of genes → Phosphoproteins → Phosphorylation on an Asp residue

Gene

Coordinates: 760,452 → 761,558

The protein

Domains

• Response regulatory domain (aa 3-120) (according to UniProt)
• HTH araC/xylS-type domain (aa 259-361) (according to UniProt)

Modification

• phosphorylated on a Asp residue by YesM

Structure

• 2JVJ (Spo0F, the Response regulatory domain, 28% identity)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: yesL-yesM-yesN
  Description: PubMed

  Regulatory mechanism

  • CcpA: repression, in CcpA regulon

Additional information

• translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

Mutant

• MGNA-A946 (yesN::erm), available at the NBRP B. subtilis, Japan
• BKE06960 (ΔyesN::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ATCAGCCAGCAGTATTTTAT, downstream forward: _UP4_TAGCCATCTCTGTTTTTTTG
• BKK06960 (ΔyesN::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ATCAGCCAGCAGTATTTTAT, downstream forward: _UP4_TAGCCATCTCTGTTTTTTTG

References

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