two-component response regulator, regulation of cold shock expression of [gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]
function
regulation of cold shock expression of [gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]
product
two-component response regulator
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.3|Lipid metabolism/ other][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.5|Cold stress proteins][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]Gene
Coordinates
2,091,705 → 2,092,304
The protein
Catalyzed reaction/ biological activity
transcription activation of the ''[gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]'' operon when phosphorylated by [protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|DesK]Paralogous protein(s)
[protein|5BB2B90D5DDD50DD9B4EF9E69925FCBFA642222B|YvfU] [SW|Domains]
[SW|Response regulatory domain] (aa 3-117) (according to UniProt)[SW|HTH luxR-type domain] (aa 131-196) (according to UniProt)Modification
phosphorylated by [protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|DesK] on an Asp residueStructure
[PDB|5IUJ] ([protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|DesK]-[protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|DesR] complex in the phosphotransfer state with low Mg2+ [20 mM]) [Pubmed|27938660][PDB|5IUK] ([protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|DesK]-[protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|DesR] complex in the phosphotransfer state with high Mg2+ [150 mM] and BeF3) [Pubmed|27938660][PDB|5IUN] ([protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|DesK]-[protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|DesR] complex in the phosphatase state) [Pubmed|27938660]Expression and Regulation
Operons
genes
[gene|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK]-[gene|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR]
description
[Pubmed|11285232]
regulation
induced by cold shock (12-fold) [Pubmed|12399512]view in new tabBiological materials
Mutant
MGNA-A320 (yocG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/320 NBRP B. subtilis, Japan]BKE19200 (Δ[gene|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCATATAAGCCATCCTTT, downstream forward: _UP4_TAAAAAAGGATCTTGGCATCBKK19200 (Δ[gene|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCATATAAGCCATCCTTT, downstream forward: _UP4_TAAAAAAGGATCTTGGCATClabs
[SW|Diego de Mendoza], Universidad Nacional de Rosario, Argentine [http://www.ibr.gov.ar/eng/investigacion/demendoza.htm homepage]References
10094672,12399512,19595746,11285232,17087771,12207704,25406381,27938660,29269314