flgM [2017-10-25 11:57:29]

anti-SigD, regulation of flagellin, motility and chemotaxis

locus: BSU35430

pI: 9.92

mw: 9.86 kDa

protein length: 88 aa

gene length: 264 bp

function: control of SigD activity

product: anti-SigD

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.2|Control of sigma factors]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.1|Signal transduction in motility and chemotaxis] → [category|SW 4.1.1.1.5|Additional chemotaxis signal transduction and regulatory proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 3,640,285 → 3,640,551

The protein

Protein family

• flgM family (according to Swiss-Prot)

Structure

• [PDB|1RP3] ([protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]-[protein|F03144BF8A187C8931938A21433431B8961E8EE7|FlgM] complex from ''Aqufex aeolicus'', 34% identity) [Pubmed|15068809]

[SW|Localization]

• secreted and extracellularly degraded by the proteases [protein|F2E3FF41357C672F80012CFE3076F890407B8B4A|Epr] and [protein|DC0FDCA3FA6742B023E6877CE9554AA1D47012BB|WprA] [Pubmed|25313396]
• secretion of FlgM requires the [protein|EB815705133E3318F655F6024B7D9BA587FD1DDA|FliF]/[protein|8DDCC3D139ACCB635BF014946E1282A72D535390|FliG] basal body proteins, the flagellar type III export apparatus components [protein|C2C67880EDF09E1BA75A4791628EE1982F9B6B0B|FliZ], [protein|6ED6C3DDC4F3142FD01D32840D955B7E4A19F385|FliP], [protein|A3C07B70C87D671979D053A6272A0FCDED6F3BB7|FliQ], [protein|9856F25F23264AD1402A85AE9E25F10B68CAD739|FliR], [protein|974FA844E263AA694477992FA50468CB8EFAB807|FlhA], and [protein|68DB2871A88535714C84FE86006AB54CEB6F0EAD|FlhB], and the substrate specificity switch regulator [protein|42277DE1030E6FEB416EAE381CD40A54D49736FF|FliK] [Pubmed|25313396]

Expression and Regulation

Operons

• genes: [gene|45F27C0789199626BA90D2C69E8F13525C911478|comFA]-[gene|D290247A43280532947B707F3AF388D7A7F404D3|comFB]-[gene|04D9D70C0BCFDCBD00B5156908C348135080A9C2|comFC]-[gene|049B1EBF64F0EBC04CE6D529C8EDB0B6B2184DF5|yvyF]-[gene|F03144BF8A187C8931938A21433431B8961E8EE7|flgM]-[gene|6F4CD36D4C1EE99EF7A503F44F53AEB8EFFEAB7F|yvyG]-[gene|767ADBEF1B40CB3C74116CEA8CFB2FAF19C1FE7B|flgK]-[gene|82AB04023BCB57967C7501E6AEAC4BB593AA6480|flgL]-[gene|E5A59D2AF53FA220BA98D9653589A8F0D24F75B8|yviE]-[gene|6A6CBD75DCC33C804F853F47D37773930D398CFE|fliW]-[gene|93F328524989597C7D2329B25E665496C9631E87|csrA]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8412657], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|8045879], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]

  regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|8412657], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]
  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P, [Pubmed|21736639], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]: repression, [Pubmed|19898538], in [regulon|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC regulon]

• genes: [gene|F03144BF8A187C8931938A21433431B8961E8EE7|flgM]-[gene|6F4CD36D4C1EE99EF7A503F44F53AEB8EFFEAB7F|yvyG]-[gene|767ADBEF1B40CB3C74116CEA8CFB2FAF19C1FE7B|flgK]-[gene|82AB04023BCB57967C7501E6AEAC4BB593AA6480|flgL]
  description: [Pubmed|21736639]

• genes: [gene|049B1EBF64F0EBC04CE6D529C8EDB0B6B2184DF5|yvyF]-[gene|F03144BF8A187C8931938A21433431B8961E8EE7|flgM]-[gene|6F4CD36D4C1EE99EF7A503F44F53AEB8EFFEAB7F|yvyG]-[gene|767ADBEF1B40CB3C74116CEA8CFB2FAF19C1FE7B|flgK]-[gene|82AB04023BCB57967C7501E6AEAC4BB593AA6480|flgL]
  description: [Pubmed|8045879]

Biological materials

Mutant

• 1A764 (no resistance), [Pubmed|8045879], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A764&Search=1A764 BGSC]
• BKE35430 (Δ[gene|F03144BF8A187C8931938A21433431B8961E8EE7|flgM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGGATTCCTCTCGCTTT, downstream forward: _UP4_AAGCAATAAAAAAGGAGAAA
• BKK35430 (Δ[gene|F03144BF8A187C8931938A21433431B8961E8EE7|flgM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGGATTCCTCTCGCTTT, downstream forward: _UP4_AAGCAATAAAAAAGGAGAAA

References

Reviews

• 25251856

Original Publications

• 8955328,19898538,8412657,8045879,20233303,10207036,8655488,23352839,21736639,25313396,26244495,15068809