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flgM [2017-10-25 11:57:29]
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flgM [2017-10-25 11:57:29]

anti-SigD, regulation of flagellin, motility and chemotaxis
Locus
BSU35430
Isoelectric point
9.92
Molecular weight
9.86 kDa
Protein length
Gene length
264 bp Sequence Blast
Function
control of SigD activity
Product
anti-SigD
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,640,285 → 3,640,551

The protein

Protein family

  • flgM family (according to Swiss-Prot)
  • Structure

  • 1RP3 (SigD-FlgM complex from Aqufex aeolicus, 34% identity) PubMed
  • Localization

  • secreted and extracellularly degraded by the proteases Epr and WprA PubMed
  • secretion of FlgM requires the FliF/FliG basal body proteins, the flagellar type III export apparatus components FliZ, FliP, FliQ, FliR, FlhA, and FlhB, and the substrate specificity switch regulator FliK PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigD: sigma factor, PubMed, in SigD regulon
  • Regulatory mechanism

  • ComK: activation, PubMed, in ComK regulon
  • DegU: activation, DegU-P, PubMed, in DegU regulon
  • ScoC: repression, PubMed, in ScoC regulon
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    Biological materials

    Mutant

  • 1A764 (no resistance), PubMed, available at BGSC
  • BKE35430 (ΔflgM::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGGATTCCTCTCGCTTT, downstream forward: _UP4_AAGCAATAAAAAAGGAGAAA
  • BKK35430 (ΔflgM::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGGATTCCTCTCGCTTT, downstream forward: _UP4_AAGCAATAAAAAAGGAGAAA
  • References

    Reviews

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    Original Publications

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