flgM

anti-SigD, regulation of flagellin, Motility and chemotaxis

Locus: BSU_35430

Isoelectric point: 9.92

Molecular weight: 9.86 kDa

Protein length: 88 aa

Gene length: 267 bp

Function: control of SigD activity

Product: anti-SigD

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → Sigma factors and their control → Control of sigma factors
• Lifestyles → Exponential and early post-exponential lifestyles → Motility and chemotaxis → Signal transduction in motility and chemotaxis → Additional chemotaxis signal transduction and regulatory proteins
• Groups of genes → Secreted proteins

Gene

Coordinates: 3,640,285 → 3,640,551

The protein

Protein family

• flgM family (single member, according to UniProt)

Structure

• 1RP3 (SigD-FlgM complex from Aqufex aeolicus, 34% identity) PubMed

Localization

• secreted and extracellularly degraded by the proteases Epr and WprA PubMed
• secretion of FlgM requires the FliF/FliG basal body proteins, the flagellar type III export apparatus components FliZ, FliP, FliQ, FliR, FlhA, and FlhB, and the substrate specificity switch regulator FliK PubMed

Expression and Regulation

Operons

• Genes: comFA-comFB-comFC-yvyF-flgM-yvyG-flgK-flgL-yviE-fliW-csrA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigD: sigma factor, PubMed, in SigD regulon

  Regulatory mechanism

  • ComK: activation, PubMed, in ComK regulon
  • DegU: activation, DegU-P, PubMed, in DegU regulon
  • ScoC: repression, PubMed, in ScoC regulon

• Genes: flgM-yvyG-flgK-flgL
  Description: PubMed

• Genes: yvyF-flgM-yvyG-flgK-flgL
  Description: PubMed

Biological materials

Mutant

• 1A764 (no resistance), PubMed, available at BGSC
• BKE35430 (ΔflgM::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGGATTCCTCTCGCTTT, downstream forward: _UP4_AAGCAATAAAAAAGGAGAAA
• BKK35430 (ΔflgM::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGGATTCCTCTCGCTTT, downstream forward: _UP4_AAGCAATAAAAAAGGAGAAA

References

Reviews

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Original Publications

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