glmM [2018-04-11 11:53:28]

phosphoglucosamine mutase, required for cell wall synthesis

Locus: BSU01770

Isoelectric point: 4.68

Molecular weight: 48.27 kDa

Protein length: 448 aa

Gene length: 1344 bp

Function: cell wall synthesis

Product: phosphoglucosamine mutase, required for cell wall synthesis

Essential: yes

E.C.: 5.4.2.10

Synonyms: ybbT

Genomic Context

Loading

Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall synthesis → Biosynthesis of peptidoglycan
• Metabolism → Additional metabolic pathways → Biosynthesis of cell wall components → Biosynthesis of peptidoglycan
• Groups of genes → Essential genes
• Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

Gene

Coordinates: 198,497 → 199,843

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• Alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate (according to Swiss-Prot)

Protein family

• phosphohexose mutase family (according to Swiss-Prot)

Modification

• phosphorylation on Ser-100 PubMed, this is an autophosphorylation PubMed

Structure

• 3PDK (from B. anthracis) PubMed

Expression and Regulation

Operons

• Genes: cdaA-cdaR-glmM-glmS
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

Biological materials

Expression vector

• pGP400: (expression in B. subtilis in pBQ200), available in Jörg Stülke's lab
• pGP1401: (expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
• pGP1402: (cloning vector for glmM (GlmM S100A) in E. coli, in pBlueskript KS), available in Jörg Stülke's lab
• pGP1403: (expression of GlmM (S100A) in B. subtilis in pBQ200), available in Jörg Stülke's lab
• pGP1405: (expression, purification of GlmM (S100A) in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab

LacZ fusion

• GP1340 (cat) based on pAC6, available in Jörg Stülke's lab

GFP fusion

• GP1382 glmM-gfp ermC (based on pGP1080), available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

FLAG-tag construct

• GP1380 lacA::cdaR-Strep aphA3 glmM-3xFLAG ermC (based on pGP1460 and pGP1087), available in Jörg Stülke's lab

Antibody

• available in Jörg Stülke's lab

References

Reviews

Loading

Original publications

Loading