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glmM

phosphoglucosamine mutase, required for cell wall synthesis

locus: BSU_01770

pI: 4.68

mw: 48.27 kDa

protein length: 448 aa

gene length: 1347 bp

function: cell wall synthesis

product: phosphoglucosamine mutase, required for cell wall synthesis

essential: yes

ec: 5.4.2.10

synonyms: ybbT

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

Gene

Coordinates: 198,497 → 199,843

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• D-glucosamine 6-phosphate --> α-D-glucosamine 1-phosphate (according to UniProt)
• inhibition of [protein|AFE2A9998219162FE32E95628B3CA9071099B61A|CdaA] activity under conditions of osmotic stress (demonstrated in L. monocytogenes ) [pubmed|32250026]

Protein family

• phosphohexose mutase family (with [protein|70412A747D1ED3E885126945DF02503667C13E5B|PgcA], [pubmed|15238632])

Modification

• phosphorylation on Ser-100 [Pubmed|17218307,17726680,20097688], this is an autophosphorylation [Pubmed|20097688]

Structure

• [PDB|3PDK] (from ''B. anthracis'') [Pubmed|21685296]

additional information

• overexpression of [protein|F022ACB2CBB8DA46B392CE1D26474093645F26DA|GlmM] suppresses the synthetic lethality of [gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA] and [gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR] inactivation [pubmed|31589605]

Expression and Regulation

Operons

• genes: [gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA]-[gene|FB23F2AB0E2A82658009D115E5876E7AF1BDE5FD|cdaR]-[gene|F022ACB2CBB8DA46B392CE1D26474093645F26DA|glmM]-[gene|4E23D2043D51C587027ABBF6335F148295E90B8F|glmS]
  description: [Pubmed|23192352]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|22211522], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Expression vectors

• pGP400: (expression in ''B. subtilis'' in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
• pGP1401: (expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
• pGP1402: (cloning vector for glmM (GlmM S100A) in ''E. coli'', in [SW|pBlueskript KS]), available in [SW|Jörg Stülke]'s lab
• pGP1403: (expression of GlmM (S100A) in ''B. subtilis'' in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
• pGP1405: (expression, purification of GlmM (S100A) in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

lacZ fusion

• GP1340 (cat) based on [SW|pAC6], available in [SW|Jörg Stülke]'s lab

GFP fusion

• GP1382 ''glmM-gfp ermC'' (based on [SW|pGP1080]), available in [SW|Jörg Stülke]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

FLAG-tag construct

• GP1380 ''lacA::cdaR-Strep aphA3 glmM-3xFLAG ermC'' (based on [SW|pGP1460] and [SW|pGP1087]), available in [SW|Jörg Stülke]'s lab

Antibody

• available in [SW|Jörg Stülke]'s lab

References

Reviews

• 28683921,18266853

Original publications

• 32250026,17726680,17218307,8550580,10231382,10671448,20097688,20525796,21685296,23192352,22211522,26240071,26585449,28516784,15238632,30668586,31589605,32250026