spmA [2019-06-06 12:32:26]

spore maturation protein (spore core dehydratation)

locus: BSU23180

pI: 10.03

mw: 21.35 kDa

protein length: 196 aa

gene length: 591 bp

function: germination

product: spore maturation protein (spore core dehydratation)

essential: no

synonyms: ypuJ

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,422,805 → 2,423,395

The protein

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|76825D77907E8CE47B17ED56D7E2A348B1ADA5EC|dacB]-[gene|F00DAFFBB136F9331C47D45BF44DC59E3EBB75FA|spmA]-[gene|6AFED2DBF443D3E7FECE211BF8FCF1FD33B7104D|spmB]
  description: [Pubmed|7642500]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190,7528199], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|15699190,7528199]

Biological materials

Mutant

• MGNA-A002 (spmA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2 NBRP B. subtilis, Japan]
• BKE23180 (Δ[gene|F00DAFFBB136F9331C47D45BF44DC59E3EBB75FA|spmA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTATATTGACCATTTTGC, downstream forward: _UP4_CGCAAAAAGAAGGGAAGGTG
• BKK23180 (Δ[gene|F00DAFFBB136F9331C47D45BF44DC59E3EBB75FA|spmA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTATATTGACCATTTTGC, downstream forward: _UP4_CGCAAAAAGAAGGGAAGGTG

References

• 7642500,7528199,15699190