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hbs [2019-02-28 10:43:46]
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hbs [2019-02-28 10:43:46]

non-specific DNA-binding protein Hbsu
locus
BSU22790
pI
9.50
mw
9.75 kDa
protein length
92 aa Sequence Blast
gene length
279 bp Sequence Blast
function
DNA packaging, function of the signal recognition complex
product
non-specific DNA-binding protein Hbsu
essential
yes
synonyms
dbpA,hupA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]

    • Gene

    Coordinates
    2,385,543 → 2,385,821

    Phenotypes of a mutant

  • essential [Pubmed|12682299,1382620]

    • The protein

    Catalyzed reaction/ biological activity

  • binds to curved DNA without apparent sequence specificity [pubmed|9894920]
  • condenses the bacterial chromosome [pubmed|9068655]
  • [protein|EFD8FC875B6787B1512C5FC4C81E7E4C9BD93028|Hbs]-mediated untwisting of the DNA stimulates [protein|C352560155F5B2E951B5059794D7B319C1A3AF2C|Nth] endonuclease activity at AP sites [Pubmed|21954439]

    • Protein family

  • DbpA subfamily (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|3FFFA28E2C16508C6A94408B739864DB7F8DD1F7|YonN]

    • Modification

  • phosphorylated on Arg-61 [Pubmed|22517742]
  • phosphorylation on Thr-4 [Pubmed|17218307]
  • can be acetylated on Lys-3, Lys-18, Lys-37, Lys-41, Lys-75, Lys-80, and Lys-86 [pubmed|27376153]

    • Structure

  • [PDB|1HUU] (Geobacillus stearothermophilus)

    • [SW|Localization]

  • associated with the nucleoid in vegetative cells and forespores [Pubmed|9068655,10715001]
  • membrane associated [Pubmed|18763711]

    • Expression and Regulation

    Operons

    genes
    [gene|EFD8FC875B6787B1512C5FC4C81E7E4C9BD93028|hbs]
    description
    [Pubmed|1902464]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1902464], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|1902464], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

    • regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]

    • regulation

  • induced by DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]) [Pubmed|16267290]

    • additional information

  • mRNA degradation is initiated by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|28977557]
  • Hbs is present with about 50,000 copies per genome in vegetative cells and spores, it is one of the most abundant proteins in B. subtilis [PubMed|10715001]
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    Biological materials

    two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab

    • labs

  • [SW|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]
  • [SW|Mohamed Marahiel], University of Marburg, Germany [http://www.uni-marburg.de/fb15/ag-marahiel/Prof.%20Dr.%20Mohamed%20A.%20Marahiel Homepage]

    • References

    Reviews

  • 11931565

    • Original publications

  • 1382620,1902464,10715001,9068655,9894920,16267290,19458035,19210617,18763711,17218307,21954439,22517742,23033921,10231583,8748031,7852372,9106208,28977557,27376153,30808761