hbs

non-specific DNA-binding protein Hbsu

locus: BSU_22790

pI: 9.50

mw: 9.75 kDa

protein length: 92 aa

gene length: 279 bp

function: DNA packaging, regulation of nucleoid compaction

product: non-specific DNA-binding protein Hbsu

essential: yes

synonyms: dbpA,hupA

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]

Gene

Coordinates: 2,385,543 → 2,385,821

Phenotypes of a mutant

• essential [Pubmed|12682299,1382620]

The protein

Catalyzed reaction/ biological activity

• binds to curved DNA without apparent sequence specificity [pubmed|9894920]
• condenses the bacterial chromosome [pubmed|9068655]
• [protein|EFD8FC875B6787B1512C5FC4C81E7E4C9BD93028|Hbs]-mediated untwisting of the DNA stimulates [protein|C352560155F5B2E951B5059794D7B319C1A3AF2C|Nth] endonuclease activity at AP sites [Pubmed|21954439]

Protein family

• bacterial histone-like protein family (with [protein|3FFFA28E2C16508C6A94408B739864DB7F8DD1F7|YonN], according to UniProt)

Paralogous protein(s)

• [protein|3FFFA28E2C16508C6A94408B739864DB7F8DD1F7|YonN]

Modification

• phosphorylated on Arg-61 [Pubmed|22517742]
• phosphorylation on Thr-4 [Pubmed|17218307]
• can be acetylated on Lys-3, Lys-18, Lys-37, Lys-41, Lys-75, Lys-80, and Lys-86 [pubmed|27376153]
• acetylation by [protein|0E6564B1B910694F28FD6EF5231E6F6A0E4BE545|YfmK] on Lys-80 inhibits DNA binding and nocleoid compaction [pubmed|30808761]
• may be acetylated by [protein|07A30814F57112196051289AA309AE6DB22CAEFF|YdgE] on Lys-41 and Lys-86 [pubmed|30808761]

Effectors of protein activity

• acetylation by [protein|0E6564B1B910694F28FD6EF5231E6F6A0E4BE545|YfmK] on Lys-80 inhibits DNA binding and nocleoid compaction [pubmed|30808761]

Structure

• [PDB|1HUU] (Geobacillus stearothermophilus) [pubmed|2508086]

[SW|Localization]

• uniformly associated with the nucleoid in vegetative cells and forespores [Pubmed|21085634,9068655,10715001]

Expression and Regulation

Operons

• genes: [gene|EFD8FC875B6787B1512C5FC4C81E7E4C9BD93028|hbs]
  description: [Pubmed|1902464]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1902464], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|1902464], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]

  regulation

  • induced by DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]) [Pubmed|16267290]

  additional information

  • mRNA degradation is initiated by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|28977557]
  • Hbs is present with about 50,000 copies per genome in vegetative cells and spores, it is one of the most abundant proteins in B. subtilis [PubMed|10715001]

Biological materials

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab

labs

• [SW|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]
• [SW|David Dubnau],Rutgers University, Newark, USA

References

Reviews

• 11931565

Original publications

• 1382620,1902464,10715001,9068655,9894920,16267290,19458035,19210617,18763711,17218307,21954439,22517742,23033921,10231583,8748031,7852372,9106208,28977557,27376153,30808761,2508086