thiW

thiamine [SW|ECF transporter] (ATP-binding protein)

locus: BSU_13220

pI: 5.71

mw: 54.48 kDa

protein length: 547 aa

gene length: 1644 bp

function: thiamine uptake

product: thiamine [SW|ECF transporter] (ATP-binding protein)

essential: no

synonyms: ykoD

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.3|ECF transporter] → [category|SW 1.2.3.3|Class I ECF transporter]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.3|Biosynthesis/ acquisition of thiamine]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,388,809 → 1,390,452

The protein

Protein family

• [SW|ABC transporter superfamily] (according to UniProt)

[SW|Domains]

• 2 [SW|ABC transporter domain]s (aa 8-250, aa 295-523) (according to UniProt)

Structure

• [PDB|4HUQ] (folate transporter from Lactobacillus brevis, 34% identity) [pubmed|23584589]

[SW|Localization]

• membrane associated (via [protein|9AF27E4CE34B5C4036840A27D93B13213E3324DD|ThiV]-[protein|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|ThiX]) [Pubmed|16291685]

Expression and Regulation

Operons

• genes: [gene|57A953B5D71F3FD8269EAF2366B8E2C7A8C79483|thiU]-[gene|9AF27E4CE34B5C4036840A27D93B13213E3324DD|thiV]-[gene|EF69891D9CF0CE57367B38FB3C69DDE1B689FCB7|thiW]-[gene|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|thiX]
  description: [Pubmed|16291685]

  regulatory mechanism

  • [protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: [SW|RNA switch], via [SW|RNA switch], in [regulon|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]

  regulation

  • repressed by thiamine ([protein|search|Thi-box]) [Pubmed|12376536]

Biological materials

Mutant

• MGNA-A755 (ykoD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/755 NBRP B. subtilis, Japan]
• BKE13220 (Δ[gene|EF69891D9CF0CE57367B38FB3C69DDE1B689FCB7|thiW]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAGCTGCTCAACCGTCAGAA, downstream forward: _UP4_ATGGCATTTCAAGAGGAGGT
• BKK13220 (Δ[gene|EF69891D9CF0CE57367B38FB3C69DDE1B689FCB7|thiW]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAGCTGCTCAACCGTCAGAA, downstream forward: _UP4_ATGGCATTTCAAGAGGAGGT

References

• 10092453,16291685,12376536,23584589