yfmT [2017-05-17 11:12:48]

vanillin dehydrogenase

locus: BSU07350

pI: 5.08

mw: 53.15 kDa

protein length: 485 aa

gene length: 1455 bp

function: unknown

product: vanillin dehydrogenase

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 807,091 → 808,548

The protein

Catalyzed reaction/ biological activity

• oxidation of vanillin to vanillic acid [Pubmed|26658822]

Paralogous protein(s)

• [protein|F9CF067A2A8E3B2BACC6A51A0E87E84640349980|AldY], [protein|5B66ADB81AB141982F187B1C5E3A9346AA064DE2|YcbD], [protein|A0BEB92D54799956A4ADE106A1388E5710141069|GabD], [protein|1ADC55F8E265AF86E29743C671CE1EA1BE7EB41E|GbsA], [protein|67A72A0EABCD807C25D8EAC61C251142B45C174E|DhaS], [protein|762718A15E5256261D79DF60F9106AF0CE2D60C6|IolA], [protein|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|PutC], [protein|69838717DC6BB27864D88C282BF5BC7CC558BFD7|RocA], [protein|F3341F205CB939498109D2A54DE842065C488DD5|AldX], [protein|99F1FAF28FB4817D94E84BD5288FA33124558933|YwdH]

Structure

• [PDB|3RHH] (from from ''Bacillus halodurans'' C-125 complexed with NADP, 32% identity, 67% similarity)

Expression and Regulation

Operons

• genes: [gene|EF0AAAF5BAE8E1F54FCA296643F7B9E3CE257B1D|yfmT]-[gene|63BCB42FC501B57ABC9FF252AC5D04BB729EA6B9|yfmS]
  description: [Pubmed|9141694,15033535]

  sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|15033535], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]

  additional information

  • in minimal medium, YfmS is present with 4,200 +/- 800 molecules per cell [PubMed|21515776]

Biological materials

Mutant

• MGNA-C232 (yfmT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2230 NBRP B. subtilis, Japan]

Labs working on this gene/protein

• [SW|Josef Altenbuchner]

References

• 9141694,15033535,26658822