gerAA

nutrient receptor, germination response to L-alanine, triggers premature germination in response to morphological defects during sporulation

Locus: BSU_33050

Isoelectric point: 8.80

Molecular weight: 53.62 kDa

Protein length: 482 aa

Gene length: 1449 bp

Function: germination response to L-alanine

Product: nutrient receptor

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Sporulation → Germination → Germinant receptors
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,390,782 → 3,392,230

Phenotypes of a mutant

• suppresses the defects of spoVV mutant spores PubMed

The protein

Catalyzed reaction/ biological activity

• triggers premature germination in response to morphological defects during sporulation PubMed

Protein family

• GerABKA family (according to UniProt)

Paralogous protein(s)

• YndD, GerBA, YfkQ, GerKA

Domains

• contains multiple membrane-spanning domains PubMed

Structure

• 6O59 (from B. megaterium, corresponds to aa 4 ... 256, 34% identity) PubMed

Localization

• outer surface of the inner spore membrane PubMed

Additional information

• 1,100 molecules are present per spore PubMed

Expression and Regulation

Operons

• Genes: gerAA-gerAB-gerAC
  Description: PubMed

  Sigma factors

  • SigG: sigma factor, PubMed, in SigG regulon
  • SigF: sigma factor, PubMed, in SigF regulon

  Regulatory mechanism

  • SpoVT: repression, PubMed, in SpoVT regulon

  Regulation

  • expressed during sporulation in the forespore (SigF, SigG, SpoVT) PubMed

  Additional information

  • 1,100 molecules are present per spore PubMed

Biological materials

Mutant

• BKE33050 (ΔgerAA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATGAGGTCACCTCTTATC, downstream forward: _UP4_AAACCAAAAGAGGTGAATAA
• BKK33050 (ΔgerAA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATGAGGTCACCTCTTATC, downstream forward: _UP4_AAACCAAAAGAGGTGAATAA

References

Reviews

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Original publications

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