tyrS

tyrosyl-tRNA synthetase (major)

locus: BSU_29670

pI: 5.21

mw: 47.57 kDa

protein length: 422 aa

gene length: 1269 bp

function: translation

product: tyrosyl-tRNA synthetase (major)

essential: yes

ec: 6.1.1.1

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 3,036,603 → 3,037,871

Phenotypes of a mutant

• essential [Pubmed|12682299]
• the gene can be deleted, but the mutant acquires suppressors in ''[gene|2D4E3D6C3634C3F42A4517D5C1A8F4F48CAFF3A6|dtrR]'' to allow expression of ''[gene|1968743631A20739B5DA8B489CB1E7E42F3B93E3|tyrZ]'' [Pubmed|25733610]
• a ''[gene|EEB0CF0AF0707AC1161F150708F7EDDB749B3C42|tyrS] [gene|1968743631A20739B5DA8B489CB1E7E42F3B93E3|tyrZ]'' double mutant is not viable [Pubmed|25733610]

The protein

Catalyzed reaction/ biological activity

• ATP + L-tyrosine + tRNA^Tyr --> AMP + diphosphate + H⁺ + L-tyrosyl-tRNA^Tyr (according to UniProt)

Protein family

• [SW|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
• [SW|Class-I aminoacyl-tRNA synthetase family] (according to UniProt)

Paralogous protein(s)

• [protein|1968743631A20739B5DA8B489CB1E7E42F3B93E3|TyrZ]

[SW|Domains]

• [SW|S4 RNA-binding domain] (aa 355-421) (according to UniProt)

Structure

• [PDB|3TS1] (complex with tyrosyl adenylate intermediate, Geobacillus stearothermophilus)
• [PDB|1JH3] (C-terminal domain, Geobacillus stearothermophilus)
• [PDB|1N53] (the [regulon|T-box|T-box] antiterminator RNA) [Pubmed|12547201]

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|D50593AB6035B7DFEC76867B4692B069D91DEC10|ytzK]-[gene|EEB0CF0AF0707AC1161F150708F7EDDB749B3C42|tyrS]
  description: [Pubmed|22383849]

  regulation

  • the [SW|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

• genes: [gene|6671E7D85E99D349679F0E9D825DC035D10FFD2E|acsA]-[gene|D50593AB6035B7DFEC76867B4692B069D91DEC10|ytzK]-[gene|EEB0CF0AF0707AC1161F150708F7EDDB749B3C42|tyrS]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|7913927], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [pubmed|7913927], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [pubmed|12618455,18083814], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

  regulation

  • repressed by glucose (4.5-fold) ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [Pubmed|7913927,12850135]

• genes: [gene|EEB0CF0AF0707AC1161F150708F7EDDB749B3C42|tyrS]
  description: [Pubmed|1735721]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1735721], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|T-box|T-box]: anti-termination, in [regulon|T-box|T-box]

  regulation

  • induced by tyrosine limitation ([regulon|T-box|T-box]) [Pubmed|19258532]
  • the [SW|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

References

Reviews

• 19258532,10546897,11163353

Original publications

• 9098057,12547201,11842119,10943892,9282752,8045882,8348614,1735721,8289305,20110252,21333656,15378759,25733610,25733611,29794222