ytpR

similar to Phe-tRNA synthetase (beta subunit)

locus: BSU_29820

pI: 4.27

mw: 21.55 kDa

protein length: 201 aa

gene length: 606 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]

Gene

Coordinates: 3,052,743 → 3,053,348

The protein

[SW|Domains]

• [SW|tRNA-binding domain] (aa 90-200) (accordinig to UniProt)

Structure

• [PDB|3BU2] (tRNA-binding protein from Staphylococcus saprophyticus, 59% identity)

Expression and Regulation

Operons

• genes: [gene|314D5C01B4BDDC3B122F6D4066647B143B2A6543|ytpP]-[gene|886EF2F8A9E031FDF919E01A858C1DBC1AFF1188|ytpQ]-[gene|EE559CD606562097F09DC627F431CA31129D7BC6|ytpR]
  description: [Pubmed|21949854]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21949854], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|21949854], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

Biological materials

Mutant

• MGNA-A512 (ytpR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/512 NBRP B. subtilis, Japan]
• BKE29820 (Δ[gene|EE559CD606562097F09DC627F431CA31129D7BC6|ytpR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGCGTTCATAAGTAAAAATC, downstream forward: _UP4_TAGACTCCCATAAATCCGCC
• BKK29820 (Δ[gene|EE559CD606562097F09DC627F431CA31129D7BC6|ytpR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGCGTTCATAAGTAAAAATC, downstream forward: _UP4_TAGACTCCCATAAATCCGCC

References

• 21949854