yhdQ [2020-04-15 16:34:06]

putative transcriptional regulator ([SW|MerR family])

locus: BSU_09560

pI: 7.89

mw: 16.25 kDa

protein length: 143 aa

gene length: 432 bp

function: unknown

product: transcriptional regulator ([SW|MerR family])

essential: no

synonyms: cueR

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 1,033,458 → 1,033,889

The protein

Protein family

• [SW|MerR family]

[SW|Domains]

• [SW|HTH merR-type domain] (aa 11-79) (according to UniProt)

Structure

• [PDB|6JGV] (from Pseudomonas putida, corresponds to aa 13 ... 115, 34% identity)

Expression and Regulation

Operons

• genes: [gene|EDFAF3AF6F2E7578733D5BF0295AD614070453F4|yhdQ]-[gene|BE0777DE7C40825D4DB7252EA84AAB3892578529|mpfA]
  description: [Pubmed|22383849]

  regulation

  • threefold induced in the presence of high Mg²⁺ concentrations [Pubmed|31964700]

Biological materials

Mutant

• MGNA-B484 (yhdQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1483 NBRP B. subtilis, Japan]
• BKE09560 (Δ[gene|EDFAF3AF6F2E7578733D5BF0295AD614070453F4|yhdQ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACGTTATAGTTATGAG, downstream forward: _UP4_TAAAACCATTTTATCTAACA
• BKK09560 (Δ[gene|EDFAF3AF6F2E7578733D5BF0295AD614070453F4|yhdQ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACGTTATAGTTATGAG, downstream forward: _UP4_TAAAACCATTTTATCTAACA

References

• 15948947,14663075,18048925,22383849