cmoO

monooxygenase, required for the conversion of S-methyl-cysteine to cysteine

Locus: BSU_29330

Isoelectric point: 5.84

Molecular weight: 36.79 kDa

Protein length: 334 aa

Gene length: 1005 bp

Function: utilization of S-methyl-cysteine

Product: monooxygenase

Essential: no

Synonyms: ytmO

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Sulfur metabolism → Conversion of S-methyl cysteine to cysteine

Gene

Coordinates: 3,003,345 → 3,004,349

Phenotypes of a mutant

• no growth with S-methyl cysteine PubMed

The protein

Protein family

• Flavin-utilizing monoxygenases

Paralogous protein(s)

• YwcH, YvbT
• YceB:

Structure

• 4US5 (from Streptomyces bottropensis, 31% identity) PubMed

Expression and Regulation

Operons

• Genes: snaA-tcyJ-tcyK-tcyL-tcyM-tcyN-cmoO-cmoI-cmoJ-ribR-sndA-ytnM
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CymR: repression, PubMed, in CymR regulon
  • AscR: activation, PubMed, in AscR regulon

  Regulation

  • induced in the presence of methionine and taurine PubMed

Biological materials

Mutant

• MGNA-A174 (ytmO::erm), available at the NBRP B. subtilis, Japan
• BKE29330 (ΔcmoO::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGGTCTAAAATACTTAAGC, downstream forward: _UP4_AAACGTTAAGGAGGGACAGA
• BKK29330 (ΔcmoO::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGGTCTAAAATACTTAAGC, downstream forward: _UP4_AAACGTTAAGGAGGGACAGA

References

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