sbcC

DNA exonuclease

Locus: BSU_10650

Isoelectric point: 5.29

Molecular weight: 128.70 kDa

Protein length: 1130 aa

Gene length: 3393 bp

Function: DNA inter-strand cross-link repair

Product: DNA exonuclease

Essential: no

Synonyms: yirY

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA repair/ recombination
• Information processing → Genetics → DNA repair/ recombination → Other proteins
• Information processing → Genetics → Genetic competence
• Lifestyles → Exponential and early post-exponential lifestyles → Genetic competence

Gene

Coordinates: 1,144,749 → 1,148,141

Phenotypes of a mutant

• reduced resistance towards electron beams PubMed

The protein

Protein family

• SMC family (with Smc, according to UniProt)

Structure

• 6S6V (from E. coli, corresponds to aa 1 ... 622, 27% identity) PubMed

Localization

• Nucleoid (Mid-cell) PubMed

Expression and Regulation

Operons

• Genes: addB-addA-sbcD-sbcC-hlpB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • ComK: activation, in ComK regulon

  Regulation

  • positive control by ComK PubMed

Biological materials

Mutant

• MGNA-B290 (yirY::erm), available at the NBRP B. subtilis, Japan
• GP894 (sbcCD::kan), available in Stülke lab PubMed
• 1A898 ( sbcC::cat), PubMed, available at BGSC
• BKE10650 (ΔsbcC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTTAATGCTTAAGGCGATCG, downstream forward: _UP4_GAGTTGATGTAAGGGAGGAG
• BKK10650 (ΔsbcC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTTAATGCTTAAGGCGATCG, downstream forward: _UP4_GAGTTGATGTAAGGGAGGAG

Labs working on this gene/protein

• Peter Graumann, Freiburg University, Germany homepage

References

Reviews

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Original publications

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