hutU

urocanase

Locus: BSU_39360

Isoelectric point: 5.66

Molecular weight: 60.43 kDa

Protein length: 552 aa

Gene length: 1659 bp

Function: histidine utilization

Product: urocanase

Essential: no

E.C.: 4.2.1.49

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of histidine

Gene

Coordinates: 4,043,574 → 4,045,232

The protein

Catalyzed reaction/ biological activity

• 4-imidazolone-5-propanoate --> H₂O + trans-urocanate (according to UniProt)

Protein family

• urocanase family (single member, according to UniProt)

Structure

• 2FKN

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: hutP-hutH-hutU-hutI-hutG-hutM
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • CodY: repression, PubMed, in CodY regulon
  • HutP: antitermination, at a protein-dependent RNA switch located between hutP and hutH PubMed, in HutP regulon

  Regulation

  • induced by histidine (HutP) PubMed

Biological materials

Mutant

• BKE39360 (ΔhutU::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AGTCATATGTTCATCCCCCT, downstream forward: _UP4_ATGCAAAAATAAAGGAGTGA
• BKK39360 (ΔhutU::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AGTCATATGTTCATCCCCCT, downstream forward: _UP4_ATGCAAAAATAAAGGAGTGA

References

Reviews

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Original publications

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