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yfhM [2019-08-27 09:34:18]
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yfhM [2019-08-27 09:34:18]

general stress protein, similar to epoxide hydrolase
Locus
BSU_08590
Isoelectric point
6.06
Molecular weight
32.61 kDa
Protein length
286 aa Sequence Blast
Gene length
861 bp Sequence Blast
Function
survival of ethanol stress
Product
unknown
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
  • Lifestyles → Coping with stress → Cell envelope stress proteins (controlled by SigM, V, W, X, Y)
  • Groups of genes → Poorly characterized/ putative enzymes

    • Gene

    Coordinates
    929,725 → 930,585

    The protein

    Protein family

  • AB hydrolase superfamily (according to UniProt)

    • Structure

  • 4NZZ (from Bacillus Megaterium, 33% identity)

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

    • Expression and Regulation

    Operons

    Genes
    yfhK-yfhL-yfhM
    Description
    PubMed

    Sigma factors

  • SigB: sigma factor, PubMed, in SigB regulon
  • SigW: sigma factor, PubMed, in SigW regulon

    • Regulation

  • induced by stress (SigB) PubMed
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    Biological materials

    Mutant

  • MGNA-C318 (yfhM::erm), available at the NBRP B. subtilis, Japan
  • BKE08590 (ΔyfhM::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCCGTTCGTATTGACAAACT, downstream forward: _UP4_TAAGCTAAAATTTCTCTCCA
  • BKK08590 (ΔyfhM::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCCGTTCGTATTGACAAACT, downstream forward: _UP4_TAAGCTAAAATTTCTCTCCA

    • References

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