recU [2017-8-11 16:0:3]

Holliday junction resolvase, DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates RecA activities, equivalent to E. coli RecU, required for efficient survival and replication restart after replication-transcription conflicts

Locus: BSU22310

Isoelectric point: 9.51

Molecular weight: 23.81 kDa

Protein length: 206 aa

Gene length: 618 bp

Function: DNA repair/ recombination and chromosome segregation

Product: Holliday junction resolvase

Essential: no

Synonyms: prfA,yppB,jopB

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA repair/ recombination
• Information processing → Genetics → DNA repair/ recombination → Double strand breaks repair
• Lifestyles → Coping with stress → Cell envelope stress proteins (controlled by SigM, V, W, X, Y)

Gene

Coordinates: 2,340,802 → 2,341,422

Phenotypes of a mutant

• the inactivations of recU and ruvA-ruvB are synthetically lethal PubMed

The protein

Catalyzed reaction/ biological activity

• binds Holliday junctions with high affinity PubMed

Effectors of protein activity

• RecU-mediated Holliday junction resolution is stimulated by RuvB-ATP PubMed

Structure

• 1ZP7 PubMed

Expression and Regulation

Operons

• Genes: recU-ponA
  Description: PubMed

  Sigma factors

  • SigM: sigma factor, PubMed, in SigM regulon
  • SigA: sigma factor, PubMed, in SigA regulon

  Regulation

  • constitutive during vegetative growth PubMed

Biological materials

Mutant

• GP891 (recU::cat), available in Jörg Stülke's lab
• BKE22310 ( recU::ermC), (available at the BGSC and in Fabian Commichau's lab) PubMed
• BP784 ( recU::ermC), (available in Fabian Commichau's lab)
• 1A895 (no resistance), PubMed, available at BGSC

References

Reviews

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Original publications

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RecU in other organisms

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