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pgk [Wed Oct 28 2015 18:25:44 GMT+0100 (CET)]
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pgk [Wed Oct 28 2015 18:25:44 GMT+0100 (CET)]

phosphoglycerate kinase, glycolytic/ gluconeogenic enzyme, universally conserved protein
locus
BSU33930
pI
4.00
mw
42.00 kDa
function
enzyme in glycolysis/ gluconeogenesis
product
phosphoglycerate kinase
essential
No
ec
2.7.2.3
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.5|ATP synthesis] → [category|SW 2.1.5.2|Substrate-level phosphorylation]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.2|Gluconeogenesis]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.5|Universally conserved proteins]

    • [SW|Categories] containing this gene/protein

  • [SW|ATP synthesis], [SW|carbon core metabolism], [SW|phosphoproteins], [SW|universally conserved proteins], [SW|most abundant proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|CggR regulon]

    • Gene

    Phenotypes of a mutant

  • essential [Pubmed|12682299], non-essential according to [Pubmed|23420519]
  • suppression of ''[protein|search|ftsZ]''(ts) mutation (reverted by addition of pyruvate) [Pubmed|24825009]

    • The protein

    Catalyzed reaction/ biological activity

  • ATP 3-phospho-D-glycerate = ADP 1,3-bisphosphoglycerate (according to Swiss-Prot)

    • Protein family

  • phosphoglycerate kinase family (according to Swiss-Prot)

    • Kinetic information

  • Two Substrate Reversible Michaelis-Menten [Pubmed|7154941]

    • [SW|Domains]

  • nucleotide binding domain (ATP) (350–353)
  • 2x substrate binding domain (21–23), (59–62)

    • Modification

  • phosphorylation on Ser-183 AND Thr-299 [Pubmed|17218307], [Pubmed|17726680]

    • [SW|Cofactors]

  • Mg2 or Mn2 [Pubmed|7154941]

    • Effectors of protein activity

  • Inhibited by Co2 , NDP and NMP [Pubmed|7154941]

    • Structure

  • [PDB|1PHP] (from ''Geobacillus stearothermophilus'')

    • Additional information

  • extensive information on the structure and enzymatic properties of Pgk can be found at [http://www.proteopedia.org/wiki/index.php/Phosphoglycerate_Kinase Proteopedia]

    • Expression and Regulation

    Operon

  • ''[protein|search|cggR]-[protein|search|gapA]-[protein|search|pgk]-[protein|search|tpiA]-[protein|search|pgm]-[protein|search|eno]'' [Pubmed|11489127]
  • ''[protein|search|pgk]-[protein|search|tpiA]-[protein|search|pgm]-[protein|search|eno]'' [Pubmed|11489127]

    • [SW|Sigma factor]

  • ''[protein|search|cggR]'': [protein|search|SigA] [Pubmed|11489127]
  • ''[protein|search|pgk]'': [protein|search|SigA] [Pubmed|11489127]

    • Regulation

  • expression activated by glucose (3.3 fold) [Pubmed|12850135]
  • ''[protein|search|cggR]'': induced by glycolytic substrates [protein|search|CggR] [Pubmed|11489127]
  • ''[protein|search|pgk]'': constitutive [Pubmed|11489127]

    • Regulatory mechanism

  • transcription repression by [protein|search|CggR] [Pubmed|11489127]

    • Additional information

  • belongs to the 100 [[Most abundant proteins]] [PubMed|15378759]

    • Biological materials

    Mutant

  • GP699 (''pgk''::''cat''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]lab
  • GP707 (''pgk''::''erm''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]

    • Expression vector

  • pGP1102 (N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP95 (N-terminal Strep-tag, in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
  • pGP91 (N-terminal Strep-tag, for [SW|SPINE], expression in ''B. subtilis'', in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab
  • pGP1513 (expression in ''B. subtilis'', in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • pGP514 (in [protein|search|pAC6]), a series of promoter deletion variants is also available in [protein|search|pAC6], available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available iin [SW|Jörg Stülke]'s lab

    • References

  • 16479537,12850135,17726680,11489127,17505547,17218307,7154941,12682299,23420519,15378759,24825009