ylmD [2017-8-11 16:0:3]

peptidoglycan-editing factor, prevents incorporation of glycine or L-serine into the PG sacculi

locus: BSU15370

pI: 6.18

mw: 30.79 kDa

protein length: 278 aa

gene length: 834 bp

function: quality control to maintain composition and integrity of peptidoglycan

product: peptidoglycan-editing factor

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]

Gene

Coordinates: 1,609,327 → 1,610,163

Phenotypes of a mutant

• sensitive to ß-lactam antibiotics [pubmed|28612943]

The protein

Protein family

• UPF0124 family (according to Swiss-Prot)

Structure

• [PDB|1T8H] (from Geobacillus stearothermophilus, 52% identity)

Expression and Regulation

Operons

• genes: [gene|ECCE99438DBFC52DA7236CB4F6486DD004CADF73|ylmD]-[gene|31789A170CB624BF8210C915F40007F802F5C81B|ylmE]-[gene|DB09F1C36257F511A84A083967A25A9D46744D14|sepF]-[gene|2B2D285D5A8F160808A21DCF07BDB924365970C5|ylmG]-[gene|BD5ACF930DD63E258D71569326752C9A1D7B9324|ylmH]
  description: [Pubmed|16420366]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

  regulation

  • repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

Biological materials

Mutant

• MGNA-B125 (ylmD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1124 NBRP B. subtilis, Japan]

References

• 14651647,16420366,28612943