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ylmD [2019-07-01 09:48:07]
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ylmD [2019-07-01 09:48:07]

peptidoglycan-editing factor, prevents incorporation of glycine or L-serine into the PG sacculi
locus
BSU15370
pI
6.18
mw
30.79 kDa
protein length
278 aa Sequence Blast
gene length
837 bp Sequence Blast
function
quality control to maintain composition and integrity of peptidoglycan
product
peptidoglycan-editing factor
essential
no
synonyms
pgeF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]

    • Gene

    Coordinates
    1,609,327 → 1,610,163

    Phenotypes of a mutant

  • sensitive to ß-lactam antibiotics [pubmed|28612943]

    • The protein

    Protein family

  • UPF0124 family (according to Swiss-Prot)

    • Structure

  • [PDB|1T8H] (from Geobacillus stearothermophilus, 52% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|ECCE99438DBFC52DA7236CB4F6486DD004CADF73|ylmD]-[gene|31789A170CB624BF8210C915F40007F802F5C81B|ylmE]-[gene|DB09F1C36257F511A84A083967A25A9D46744D14|sepF]-[gene|2B2D285D5A8F160808A21DCF07BDB924365970C5|ylmG]-[gene|BD5ACF930DD63E258D71569326752C9A1D7B9324|ylmH]
    description
    [Pubmed|16420366]

    regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

    • regulation

  • repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]
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    Biological materials

    Mutant

  • MGNA-B125 (ylmD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1124 NBRP B. subtilis, Japan]
  • BKE15370 (Δ[gene|ECCE99438DBFC52DA7236CB4F6486DD004CADF73|ylmD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGATCCAACCTTTCG, downstream forward: _UP4_GGAATGAAGGAGGCATAAAA
  • BKK15370 (Δ[gene|ECCE99438DBFC52DA7236CB4F6486DD004CADF73|ylmD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGATCCAACCTTTCG, downstream forward: _UP4_GGAATGAAGGAGGCATAAAA

    • References

  • 14651647,16420366,28612943