glpT

glycerol-3-phosphate permease

Locus: BSU_02140

Isoelectric point: 9.37

Molecular weight: 49.64 kDa

Protein length: 444 aa

Gene length: 1335 bp

Function: glycerol-3-phosphate uptake

Product: glycerol-3-phosphate permease

Essential: no

Synonyms: ybeE

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Carbohydrate transporter
• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of glycerol/ glycerol-3-phosphate
• Metabolism → Lipid metabolism → Utilization of lipids → Utilization of phospholipids
• Groups of genes → Membrane proteins

Gene

Coordinates: 233,994 → 235,328

The protein

Protein family

• major facilitator superfamily (according to UniProt)

Structure

• 1PW4 (from E. coli, 60% identity) PubMed

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: glpT-glpQ
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • GlpP: antitermination, via a protein-dependent RNA switch PubMed, in GlpP regulon
  • PhoP: activation, PubMed, in PhoP regulon
  • CcpA: repression, in CcpA regulon

  Regulation

  • induced by glycerol (GlpP) PubMed

Biological materials

Mutant

• QB5437 (ermC), available in the Stülke lab
• BKE02140 (ΔglpT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTAATAATTATCCCCCTT, downstream forward: _UP4_TAAGAAAGACACATAAAAGA
• BKK02140 (ΔglpT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTAATAATTATCCCCCTT, downstream forward: _UP4_TAAGAAAGACACATAAAAGA

References

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