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ykuP [2018-10-11 18:39:21]
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ykuP [2018-10-11 18:39:21]

flavodoxin, binds FMN, replaces ferredoxin under conditions of iron limitation
Locus
BSU14170
Isoelectric point
4.10
Molecular weight
20.11 kDa
Protein length
178 aa Sequence Blast
Gene length
534 bp Sequence Blast
Function
electron transfer
Product
flavodoxin
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,488,413 → 1,488,868

The protein

Protein family

  • flavodoxin-like domain (according to Swiss-Prot)
  • Paralogous protein(s)

    Cofactors

  • FMN
  • Structure

  • 6FSG (from B. cereus, 46% identity)
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigI: sigma factor, PubMed, in SigI regulon
  • Regulatory mechanism

  • ResD: activation, PubMed, in ResD regulon
  • Kre: repression, in Kre regulon
  • NsrR: repression, PubMed, in NsrR regulon
  • Fur: repression, PubMed, in Fur regulon
  • Regulation

  • expressed under anaerobic conditions (ResD) PubMed
  • induced by iron starvation (second wave) (Fur) PubMed
  • expression of the operon is strongly induced at the beginning of biofilm formation PubMed
  • Additional information

  • the YkuN-YkuO-YkuP operon is strongly unregulated in a kre mutant PubMed
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    Biological materials

    Mutant

  • MGNA-B339 (ykuP::erm), available at the NBRP B. subtilis, Japan
  • BKE14170 (ΔykuP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTCCTTGTTCCTCCTCT, downstream forward: _UP4_TGATCACTCACTGGGAACTG
  • BKK14170 (ΔykuP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTCCTTGTTCCTCCTCT, downstream forward: _UP4_TGATCACTCACTGGGAACTG
  • References

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