grpE

heat-shock protein (activation of [protein|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|DnaK])

locus: BSU_25480

pI: 4.26

mw: 21.54 kDa

protein length: 187 aa

gene length: 564 bp

function: control of [protein|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|DnaK] activity

product: heat-shock protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.2|Chaperones/ protein folding]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]

Gene

Coordinates: 2,627,971 → 2,628,534

The protein

Protein family

• grpE family (single member, according to UniProt)

Structure

• [PDB|4ANI] ([protein|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|DnaK]-[protein|ECAB685E9038DC03FA2CC659112BB07D30DE5C8C|GrpE] complex from ''Geobacillus kaustophilus'', 70% identity) [Pubmed|22544739]

[SW|Localization]

• Cytoplasm (Homogeneous) [Pubmed|16479537]

Expression and Regulation

Operons

• genes: [gene|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|hrcA]-[gene|ECAB685E9038DC03FA2CC659112BB07D30DE5C8C|grpE]-[gene|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|dnaK]-[gene|D4C75B39B2DAECB9D85DAD66AAD4F923C9AEBEA7|dnaJ]-[gene|70D717806F5174447E01FF33DC8299CB025888CD|yqeT]-[gene|F8DC71B29D9C9BF4CF5124D0F01A71548306B9F9|yqeU]-[gene|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|yqeV]
  description: [Pubmed|9023197]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1339421], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|HrcA]: repression, [Pubmed|1339421], in [regulon|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|HrcA regulon]

  regulation

  • induced by heat shock ([protein|search|HrcA]) [Pubmed|1339421]

Biological materials

Mutant

• BKE25480 (Δ[gene|ECAB685E9038DC03FA2CC659112BB07D30DE5C8C|grpE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTGTTCACCTCCCTCA, downstream forward: _UP4_TAATTACATAGCAGGAGGTT
• BKK25480 (Δ[gene|ECAB685E9038DC03FA2CC659112BB07D30DE5C8C|grpE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTGTTCACCTCCCTCA, downstream forward: _UP4_TAATTACATAGCAGGAGGTT

labs

• [SW|Wolfgang Schumann], Bayreuth University, Germany [http://www.genetik.uni-bayreuth.de/LSGenetik1/schumann_research.htm Homepage]

References

• 10383760,9023197,1339421,16479537,1339421,7592421,22544739