padC [2019-07-12 08:07:06]

phenolic acid decarboxylase

locus: BSU34400

pI: 4.87

mw: 18.93 kDa

protein length: 161 aa

gene length: 486 bp

function: resistance to salicylic acid

product: phenolic acid decarboxylase

essential: no

synonyms: pad, yveH

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.10|Resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)]

Gene

Coordinates: 3,532,325 → 3,532,810

The protein

Catalyzed reaction/ biological activity

• conversion of ferulic acid to 4-vinylguaiacol [Pubmed|26658822]

Protein family

• padC family (according to Swiss-Prot)

Structure

• [PDB|2P8G]

Expression and Regulation

Operons

• genes: [gene|1E0BA378F14ADF03D613DA3BF3241C1ADE75075E|yveF]-[gene|1F56C126099E1D5241069E06477F277C12B20C58|yveG]-[gene|ECA64CB1E4BF8B6B0854B1B63C9E85A519059C6C|padC]
  description: [Pubmed|18326577]

  regulatory mechanism

  • [protein|A7824EACE0C109C676A374FD21F59D671B703305|PadR]: repression, [Pubmed|18326577], in [regulon|A7824EACE0C109C676A374FD21F59D671B703305|PadR regulon]

  regulation

  • induced by phenolic acid ([protein|search|PadR]) [Pubmed|18326577]

Biological materials

Mutant

• BKE34400 (Δ[gene|ECA64CB1E4BF8B6B0854B1B63C9E85A519059C6C|padC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTACACACTCTCCTTA, downstream forward: _UP4_TAATAAACATAAAAACCCGG
• BKK34400 (Δ[gene|ECA64CB1E4BF8B6B0854B1B63C9E85A519059C6C|padC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTACACACTCTCCTTA, downstream forward: _UP4_TAATAAACATAAAAACCCGG

References

• 18326577,17295427,9546183,21685295,26408050,23081771,21045284,26190963,26658822