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mutM [2020-03-03 11:57:54]
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mutM [2020-03-03 11:57:54]

formamidopyrimidine-DNA glycosidase
locus
BSU_29080
pI
8.89
mw
31.06 kDa
protein length
276 aa Sequence Blast
gene length
831 bp Sequence Blast
function
DNA repair
product
formamidopyrimidine-DNA glycosidase
essential
no
ec
3.2.2.23
synonyms
ytaE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.5|Oxidized guanine (GO) DNA repair system]

    • Gene

    Coordinates
    2,972,329 → 2,973,159

    Phenotypes of a mutant

  • increased susceptibility to Cr(VI) due to the accumulation of oxidative DNA damage [Pubmed|24973075]
  • absence of MutM promotes mutagenesis allowing nutritionally stressed ''B. subtilis'' cells to escape from growth limiting conditions [Pubmed|25825434]
  • increased sponteous mutation rate [Pubmed|25825434]

    • The protein

    Catalyzed reaction/ biological activity

  • Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine (according to UniProt)
  • The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate (according to UniProt)

    • Protein family

  • FPG family (single member, according to UniProt)

    • Structure

  • [PDB|2F5S] (complex with oxoG:C, Geobacillus stearothermophilus)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA]-[gene|EC96EDED2472607DFA4C5EBA054157F7832EE41C|mutM]-[gene|3BEE8E248BC583FCCB37B7A209A122DD93CD398C|ytaF]-[gene|E163A282560DB882B81E783E7E95E1960F4AE4D2|ytaG]
    description
    [pubmed|22383849]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|23396918], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
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    Biological materials

    Mutant

  • MGNA-A116 (mutM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/116 NBRP B. subtilis, Japan]
  • BKE29080 (Δ[gene|EC96EDED2472607DFA4C5EBA054157F7832EE41C|mutM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATCCATCACTTCCTATC, downstream forward: _UP4_TAGCATAATGCTTCATTGTC
  • BKK29080 (Δ[gene|EC96EDED2472607DFA4C5EBA054157F7832EE41C|mutM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATCCATCACTTCCTATC, downstream forward: _UP4_TAGCATAATGCTTCATTGTC

    • References

    Reviews

  • 22933559

    • Original publications

  • 19011023,19889642,24973075,25825434